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DNA from archived dried blood spot
Optimization of extraction of genomic DNA from archived dried blood spot (DBS): potential software in epidemiological analysis & bio banking.
Background: Restricted infrastructure is out there to gather, retailer and transport venous blood in area epidemiological research. Dried blood spot (DBS) is a sturdy potential various pattern supply for epidemiological research & bio banking. A steady supply of genomic DNA (gDNA) is required for long run storage in bio financial institution for its downstream functions. Our goal is to optimize the strategies of gDNA extraction from saved DBS and with the goal of unveiling its utility in giant scale epidemiological research.
Strategies: The aim of this research was to extract the utmost quantity of gDNA from DBS on Whatman 903 protein saver card. gDNA was extracted by way of column (Qiagen) & magnetic bead based mostly (Invitrogen) strategies. Quantification of extracted gDNA was carried out with a spectrophotometer, fluorometer, and integrity analyzed by agarose gel electrophoresis.
End result: Giant variation was noticed in amount & purity (260/280 ratio, 1.8-2.9) of the extracted gDNA. The intact gDNA bands on the electrophoresis gel mirror the robustness of DBS for gDNA even after extended storage time. The extracted gDNA quantity 2.16 – 24 ng/µl is adequate for its PCR based mostly downstream software, however sadly it could possibly’t be used for entire genome sequencing or genotyping from extracted gDNA. Sequencing or genotyping could be achieved by after growing template copy quantity by way of entire genome amplification of extracted gDNA. The obtained outcomes create a base for future analysis to develop high-throughput analysis and extraction strategies from blood samples.
Conclusion: The above outcomes reveal, DBS could be utilized as a possible and sturdy pattern supply for bio banking in area epidemiological research.
Anti-CNG-1 Antibody |
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| A05494-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-Periphilin 1 Antibody |
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| A06996-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse. |
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Anti-Lyl-1 Antibody |
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| A07491-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat. |
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Anti-GLI-1 Antibody |
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| A07972-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-Cerebellin 1 Antibody |
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| A09176-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-DOC-1 Antibody |
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| A09467-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse. |
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Anti-Bag-1 Antibody |
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| A02423-1 | BosterBio | 50 ul | EUR 397 |
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Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat. |
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Anti-TUB 1 Antibody |
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| A02917-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-Dok-1 Antibody |
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| A03039-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat. |
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Anti-NPDC-1 Antibody |
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| A12846-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-Apaf-1 (human) Monoclonal Antibody (2E12) |
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| M00889-1 | BosterBio | 100ug | EUR 432 |
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Description: Rat Monoclonal Apaf-1 (human) Antibody (2E12). Validated in ELISA, IP, IF, WB and tested in Human. |
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Anti-ALK-1 antibody |
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| STJ96547 | St John's Laboratory | 200 µl | EUR 197 |
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Description: Rabbit polyclonal to ALK-1. |
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Anti-ALK-1 antibody |
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| STJ91563 | St John's Laboratory | 200 µl | EUR 197 |
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Description: Rabbit polyclonal to ALK-1. |
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Anti-HO-1 Monoclonal Antibody (HO-1-2) |
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| M00253-1 | BosterBio | 1mg | EUR 397 |
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Description: Mouse Monoclonal HO-1 Antibody (HO-1-2). Validated in Flow Cytometry, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Esrp-1 Monoclonal Antibody |
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| M06068-1 | BosterBio | 100uL | EUR 443 |
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Description: Mouse Monoclonal Esrp-1 Antibody. Validated in WB and tested in Human. |
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Anti-Presenilin 1 Monoclonal Antibody |
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| M00138-1 | BosterBio | 100ug | EUR 397 |
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Description: Rabbit Monoclonal Presenilin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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Anti-Galectin 1 Monoclonal Antibody |
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| M00470-1 | BosterBio | 100ug | EUR 397 |
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Description: Rabbit Monoclonal Galectin 1 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat. |
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Anti-DJ-1 Monoclonal Antibody |
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| M00757-1 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal DJ-1 Antibody. Validated in IHC, WB and tested in Bovine, Human. |
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Anti-Enolase 1 Monoclonal Antibody |
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| M01250-1 | BosterBio | 100ul | EUR 397 |
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Description: Anti-Enolase 1 Monoclonal Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse, cow, pig, horse |
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Anti-Dynamin 1 Monoclonal Antibody |
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| M02536-1 | BosterBio | 100ug | EUR 397 |
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Description: Rabbit Monoclonal Dynamin 1 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
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Anti-Angiopoietin 1/ANGPT1 Antibody |
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| PA1333-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Galectin 1/LGALS1 Antibody |
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| PB9240-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-TNF Receptor 1 Antibody |
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| A00294-1 | BosterBio | 200ug | EUR 522 |
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Description: Rabbit Polyclonal TNF Receptor 1 Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Galectin 1/Lgals1 Antibody |
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| A00470-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-LOX-1/OLR1 Antibody |
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| A00760-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-Syndecan-1/SDC1 Antibody |
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| A00991-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-IRAK-1/IRAK1 Antibody |
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| A01021-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Neurexin 1/NRXN1 Antibody |
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| A01490-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Hexokinase 1/HK1 Antibody |
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| A01504-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Musashi 1/Msi1 Antibody |
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| A05052-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Pyrophosphatase 1/PPA1 Antibody |
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| A07485-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-TCP-1 eta Antibody |
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| A08169-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for TCP-1 eta Antibody (CCT7) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-Relaxin 1/RLN1 Antibody |
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| A08367-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-TCP-1 zeta Antibody |
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| A09373-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for TCP-1 zeta Antibody (CCT6A) detection.tested for WB in Human, Mouse, Rat. |
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Anti-Synaptotagmin 1/SYT1 Antibody |
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| A02314-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Syntenin-1 Monoclonal Antibody |
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| A02475-1 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal Antibody for Syntenin-1 Antibody (SDCBP) detection. Tested with WB in Human, Rat, Dog, Pig. |
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Anti-Dsg1/Desmoglein 1 Antibody |
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| A02655-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Dsg1 Antibody (DSG1) detection.tested for IHC, WB in Human, Mouse, Rat. |
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Anti-CAF-1 p150 Antibody |
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| A02732-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for CAF-1 p150 Antibody (CHAF1A) detection. Tested with WB in Human, Mouse. |
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Anti-Talin 1/TLN1 Antibody |
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| A02859-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Islet 1/ISL1 Antibody |
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| A02969-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-CHST8/Galnac4St 1 Antibody |
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| A10989-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal CHST8/Galnac4St 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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Anti-P-glycoprotein (human) Monoclonal Antibody (JSB-1) |
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| M00049-1 | BosterBio | 125ug | EUR 586 |
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Description: Mouse Monoclonal P-glycoprotein (human) Antibody (JSB-1). Validated in IF and tested in Human. |
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Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 |
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| M04431-1 | BosterBio | 100uL | EUR 385 |
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Description: Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 tested in WB, IHC, reactive to Human |
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AXMIR-1 RNA oligo anti-miRNA-1-3p with Xmotif |
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| AXMIR-1 | SBI | 10 reactions | EUR 438 |
Anti-Sumo 1 Rabbit Monoclonal Antibody |
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| M00631-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal Sumo 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Cytokeratin 1 Rabbit Monoclonal Antibody |
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| M01639-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal Cytokeratin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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Anti-Mitofusin 1 Antibody (monoclonal, 3H3) |
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| M02172-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-Phospho-Beclin-1 (Ser295) Antibody |
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| P00327-1 | BosterBio | 100ul | EUR 398 |
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Description: Rabbit Polyclonal Phospho-Beclin-1 (Ser295) Antibody. Validated in WB and tested in Human. |
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Anti-Phospho-Raf-1 (Ser642) Antibody |
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| P00446-1 | BosterBio | 100ul | EUR 398 |
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Description: Rabbit Polyclonal Phospho-Raf-1 (Ser642) Antibody. Validated in WB and tested in Human, Rat. |
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Anti-Integrin alpha 1/ITGA1 Antibody |
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| PA1045-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Caspase-1(P20)/CASP1 Antibody |
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| PA1440-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-VEGF Receptor 1/FLT1 Antibody |
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| PA1966-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-HIF-1 alpha/HIF1A Antibody |
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| A00013-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-Cleaved-Notch 1 (V1754) Antibody |
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| A00033-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Cleaved-Notch 1 (V1754) Antibody (NOTCH1) detection.tested for WB in Human, Mouse, Rat. |
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Anti-HDAC-1 (C-terminus) Antibody |
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| A00256-1 | BosterBio | 100uL | EUR 443 |
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Description: Rabbit Polyclonal HDAC-1 (C-terminus) Antibody. Validated in IF, IHC, WB and tested in Human. |
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Anti-VEGF Receptor 1/FLT1 Antibody |
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| A00534-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-Liver Carboxylesterase 1/CES1 Antibody |
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| A01741-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Hyaluronan synthase 1/HAS1 Antibody |
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| A04784-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-CELSR3/Flamingo Homolog 1 Antibody |
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| A07204-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal CELSR3/Flamingo Homolog 1 Antibody. Validated in IF and tested in Human, Mouse, Rat. |
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Anti-LPCAT2/Acyltransferase Like 1 Antibody |
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| A07471-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal LPCAT2/Acyltransferase Like 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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Anti-EBP50/NHERF-1/SLC9A3R1 Antibody |
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| A02427-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-IL1R2/Il 1 Rii Antibody |
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| A03106-1 | BosterBio | 1 ml | EUR 397 |
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Description: Rabbit Polyclonal IL1R2/Il 1 Rii Antibody. Validated in IP, WB and tested in Human. |
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Anti-TTF-1 |
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| DB-089-1 | DB Biotech | 1 ml | EUR 750 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-HLA-G (human) Monoclonal Antibody (MEM-G/1) |
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| M01235-1 | BosterBio | 100ug | EUR 397 |
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Description: Mouse Monoclonal HLA-G (human) Antibody (MEM-G/1). Validated in IHC, WB and tested in Human. |
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Endothelin-1 (1-15), amide, human |
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| A1111-1 | ApexBio | 1 mg | EUR 722 |
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Description: Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium and have a key role in vascular homeostasis. |
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Haptoglobin, (Phenotype 1-1) Human Plasma |
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| 7536-1 | Biovision | EUR 321 | |
Hemokinin 1 (human) |
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| B5318-1 | ApexBio | 1 mg | EUR 340 |
Anti-Musashi 1/Msi1 Antibody (monoclonal, 2B9) |
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| M05052-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Visinin-like Protein 1 Monoclonal Antibody |
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| M06959-1 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal Visinin-like Protein 1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody |
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| M00066-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal HMGB1/Hmg 1 Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
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Anti-p27 KIP 1 Rabbit Monoclonal Antibody |
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| M00173-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Anti-p27 KIP 1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Rat |
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Anti-Caveolin-1/CAV1 Antibody (monoclonal, 12C7) |
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| M00179-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-MHC class 1 Rabbit Monoclonal Antibody |
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| M00194-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal MHC class 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human. |
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Anti-Integrin beta 1 Rabbit Monoclonal Antibody |
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| M00772-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal Integrin beta 1 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
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Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody |
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| M01106-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal ARG1/Arginase 1 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat. |
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Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody |
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| M02033-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal MLANA/Mart 1 Antibody. Validated in IF, WB and tested in Human. |
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Anti-TCP-1α Monoclonal Antibody (91a) |
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| M02389-1 | BosterBio | 200ug | EUR 498 |
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Description: Rat Monoclonal TCP-1α Antibody (91a). Validated in Flow Cytometry, IP, IF, WB and tested in Human, Mouse, Rat. |
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Anti-DSG1/Desmoglein 1 Rabbit Monoclonal Antibody |
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| M02655-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal DSG1/Desmoglein 1 Antibody. Validated in IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
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Anti-Phospho-Glycogen Synthase 1 (S645) Antibody |
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| P03512-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Phospho-Glycogen Synthase 1 (S645) Antibody (GYS1) detection.tested for WB in Human, Mouse, Rat. |
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Anti-Angiotensin Converting Enzyme 1/ACE Antibody |
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| PA2196-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-GABA B Receptor 1/GABBR1 Antibody |
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| A07297-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-Vangl1/Vang Like Protein 1 Antibody |
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| A07587-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Vangl1 Antibody (VANGL1) detection. Tested with WB in Human, Mouse. |
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Anti-E74 like factor 1/ELF1 Antibody |
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| A03187-1 | BosterBio | 100ug/vial | EUR 294 |
Neuregulin/Heregulin-1? (NRG-1?/HRG-1?), human recombinant protein |
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| P1054-1 | ApexBio | 1 mg | EUR 3947 |
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Description: Neuregulin (NRG) is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells and plays an important role in heart structure and function through inducing cardiomyocyte differentiation |
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miRZip-1 anti-miR-1 microRNA construct |
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| MZIP1-PA-1 | SBI | Bacterial Streak | EUR 684 |
Anti-EBV/LMP-1 |
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| DB-060-1 | DB Biotech | 1 ml | EUR 750 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-EBV/LMP-1 |
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| DB-061-1 | DB Biotech | 1 ml | EUR 750 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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MMP-1 Matrix Metalloproteinase-1 Human Recombinant Protein |
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| PROTP03956-1 | BosterBio | Regular: 20ug | EUR 317 |
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Description: MMP 1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 393 amino acids (100-469a.a) and having a molecular mass of 45kDa. MMP 1 is fused to a 23 amino acid His-tag at N-terminus. |
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Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody |
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| M00522-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal AFP/Alpha 1 Fetoprotein Antibody. Validated in IP, IF, WB and tested in Human. |
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Comparability of 11 strategies for genomic DNA extraction appropriate for large-scale whole-genome genotyping and long-term DNA banking utilizing blood samples.
Over the latest years, subsequent technology sequencing and microarray applied sciences have revolutionized scientific analysis with their functions to high-throughput evaluation of organic methods. Isolation of excessive portions of pure, intact, double stranded, extremely concentrated, not contaminated genomic DNA is prerequisite for profitable and dependable giant scale genotyping evaluation.
Excessive portions of pure DNA are additionally required for the creation of DNA-banks. Within the current research, eleven totally different DNA extraction procedures, together with phenol-chloroform, silica and magnetic beads based mostly extractions, have been examined to determine their relative effectiveness for extracting DNA from ovine blood samples.
The standard and amount of the differentially extracted DNA was subsequently assessed by spectrophotometric measurements, Qubit measurements, real-time PCR amplifications and gel electrophoresis.
Processing time, depth of labor and value for every methodology have been additionally evaluated. Outcomes revealed vital variations among the many eleven procedures and solely 4 of the strategies yielded passable outputs.
These 4 strategies, comprising three modified silica based mostly business kits (Modified Blood, Modified Tissue, Modified Dx kits) and an in-house developed magnetic beads based mostly protocol, have been most acceptable for extracting prime quality and amount DNA appropriate for large-scale microarray genotyping and in addition for long-term DNA storage as demonstrated by their profitable software to 600 people.
DNA barcoding for conservation, seed banking and ecological restoration of Acacia within the Midwest of Western Australia.
We used DNA barcoding to deal with an essential conservation concern within the Midwest of Western Australia, engaged on Australia’s largest genus of flowering plant.
We examined whether or not or not at the moment advisable plant DNA barcoding areas (matK and rbcL) have been capable of discriminate Acacia taxa of various phylogenetic distances, and finally determine an ambiguously labelled seed assortment from a mine-site restoration venture.
Though matK efficiently recognized the unknown seed because the uncommon and conservation precedence listed A. karina, and was capable of resolve six of the eleven research species, this area was tough to amplify and sequence.
In distinction, rbcL was simple to get well and align, however couldn’t decide the origin of the seed and solely resolved three of the 11 species. Different chloroplast areas (rpl32-trnL, psbA-trnH, trnL-F and trnK) had combined success resolving the studied taxa. Usually, species have been higher resolved in multilocus knowledge units in comparison with single-locus knowledge units.
We advocate utilizing the formal barcoding areas supplemented with knowledge from different plastid areas, significantly rpl32-trnL, for barcoding in Acacia. Our research demonstrates the novel use of DNA barcoding for seed identification and illustrates the sensible potential of DNA barcoding for the rising self-discipline of restoration ecology.
Influenza A H3 Antibody |
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| abx021978-1mg | Abbexa | 1 mg | EUR 878 |
Lung Tissue Lysate (Tumor) |
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| 1703-07 | ProSci | 0.1 mg | EUR 280.25 |
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Description: Lung tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Lysate (Tumor) |
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| 1707-07 | ProSci | 0.1 mg | EUR 280.25 |
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Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Breast Tissue Lysate (Normal) |
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| 1710-07 | ProSci | 0.1 mg | EUR 217.25 |
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Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Breast Tissue Lysate (Tumor) |
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| 1714-07 | ProSci | 0.1 mg | EUR 280.25 |
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Description: Breast tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Colon Tissue Lysate (Normal) |
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| 1715-07 | ProSci | 0.1 mg | EUR 217.25 |
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Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide |
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| 11-563 | ProSci | 0.05 mg | EUR 527 |
|
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide |
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Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide |
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| 11-564 | ProSci | 0.05 mg | EUR 527 |
|
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide |
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Influenza A antibody |
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| 10-1096 | Fitzgerald | 500 ug | EUR 208 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1097 | Fitzgerald | 1 mg | EUR 349 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1098 | Fitzgerald | 1 mg | EUR 605 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1099 | Fitzgerald | 1 mg | EUR 605 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1100 | Fitzgerald | 500 ug | EUR 349 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1101 | Fitzgerald | 1 mg | EUR 605 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1102 | Fitzgerald | 500 ug | EUR 349 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1103 | Fitzgerald | 500 ug | EUR 349 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1241 | Fitzgerald | 500 ug | EUR 349 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-1243 | Fitzgerald | 500 ug | EUR 349 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
|||
| 10-7866 | Fitzgerald | 1 mg | EUR 403 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-7867 | Fitzgerald | 1 mg | EUR 403 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50A | Fitzgerald | 1 mg | EUR 503 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50B | Fitzgerald | 1 mg | EUR 392 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50C | Fitzgerald | 1 mg | EUR 392 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50D | Fitzgerald | 1 mg | EUR 279 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50E | Fitzgerald | 1 mg | EUR 279 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50F | Fitzgerald | 1 mg | EUR 273 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50G | Fitzgerald | 1 mg | EUR 408 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50H | Fitzgerald | 1 mg | EUR 541 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50I | Fitzgerald | 1 mg | EUR 408 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50K | Fitzgerald | 1 mg | EUR 273 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50L | Fitzgerald | 1 mg | EUR 300 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50M | Fitzgerald | 1 mg | EUR 300 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50N | Fitzgerald | 1 mg | EUR 300 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50O | Fitzgerald | 1 mg | EUR 300 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50P | Fitzgerald | 1 mg | EUR 300 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50Q | Fitzgerald | 1 mg | EUR 273 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50R | Fitzgerald | 1 mg | EUR 273 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50S | Fitzgerald | 1 mg | EUR 273 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50T | Fitzgerald | 1 mg | EUR 273 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A antibody |
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| 10-I50U | Fitzgerald | 1 mg | EUR 273 |
|
Description: Mouse monoclonal Influenza A antibody |
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Influenza A virus H3 PCR kit |
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| PCR-H649-48R | Bioingentech | 50T | EUR 823 |
Influenza A virus H3 PCR kit |
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| PCR-H649-96R | Bioingentech | 100T | EUR 1113 |
Influenza A Virus H3 Antigen Antibody |
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| abx415738-01mg | Abbexa | 0.1 mg | EUR 718 |
Influenza A virus fragment |
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| A1075-10 | ApexBio | 10 mg | EUR 282 |
|
Description: Influenza A virus fragment has the RNA sequence of Leu-Lys-Phe-Ala-Phe-Ser-Met-Met.Influenzavirus A is agenusof the Orthomyxoviridaefamily of viruses. |
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IAV H3N2 nucleoprotein (A/Brisbane/10/2007) [His] |
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| DAG2329 | Creative Diagnostics | 100 µg | EUR 724 |
Influenza B Virus Florida 07/04 |
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| 7-07918 | CHI Scientific | 10µg | Ask for price |
Influenza B Virus Florida 07/04 |
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| 7-07919 | CHI Scientific | 50µg | Ask for price |
Influenza B Virus Florida 07/04 |
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| 7-07920 | CHI Scientific | 1mg | Ask for price |
Mouse Monoclonal Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG |
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| H3N21-M | Alpha Diagnostics | 100 ul | EUR 482 |
Influenza A virus H3 RT PCR kit |
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| RTq-H649-100R | Bioingentech | 100T | EUR 1311 |
Influenza A virus H3 RT PCR kit |
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| RTq-H649-150R | Bioingentech | 150T | EUR 1787 |
Influenza A virus H3 RT PCR kit |
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| RTq-H649-50R | Bioingentech | 50T | EUR 963 |
Influenza A HA H1 antibody |
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| 10-7839 | Fitzgerald | 500 ug | EUR 291 |
|
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody |
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Influenza A HA H1 antibody |
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| 10-7840 | Fitzgerald | 500 ug | EUR 291 |
|
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody |
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Influenza A HA H5 antibody |
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| 10-7854 | Fitzgerald | 1 mg | EUR 732 |
|
Description: Mouse monoclonal Influenza A HA H5 antibody |
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Influenza A HA H5 antibody |
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| 10-7855 | Fitzgerald | 1 mg | EUR 732 |
|
Description: Mouse monoclonal Influenza A HA H5 antibody |
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Influenza A HA H5 antibody |
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| 10-7856 | Fitzgerald | 1 mg | EUR 732 |
|
Description: Mouse monoclonal Influenza A HA H5 antibody |
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Influenza B (B / Florida / 07 / 04) Protein |
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| abx060630-1mg | Abbexa | 1 mg | EUR 1873 |
Influenza B Virus Florida 07 / 04 Protein |
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| 20-abx260511 | Abbexa |
|
|
Influenza A virus H3 One-Step PCR kit |
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| Oneq-H649-100R | Bioingentech | 100T | EUR 1610 |
Influenza A virus H3 One-Step PCR kit |
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| Oneq-H649-150R | Bioingentech | 150T | EUR 2205 |
Influenza A virus H3 One-Step PCR kit |
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| Oneq-H649-50R | Bioingentech | 50T | EUR 1175 |
IAV H3N2 H3 (aa 17-529) (A/Panama/07/99) [His] |
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| DAG2162 | Creative Diagnostics | 100 µg | Ask for price |
hemagglutinin (332-340) [Influenza A virus] |
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| A1141-10 | ApexBio | 10 mg | EUR 340 |
|
Description: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. |
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IAV H3N2 (aa 18-530) (A/Brisbane/10/2007) [His] |
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| DAG1693 | Creative Diagnostics | 50 ug | EUR 1681 |
IAV H3N2 non-structural 1 (A/Brisbane/10/2007 ) [His] |
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| DAG2335 | Creative Diagnostics | 100 µg | EUR 724 |
Recombinant (HEK) Influenza A HA1 protein (A/California/07/2009 (H1N1; 18-345 aa,55-60 kda, >95%, his-tag) |
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| H1N1-02-R-10 | Alpha Diagnostics | 10 ug | EUR 286 |
Recombinant Influenza H3N2 Brisbane Protein, Untagged, Insect-10ug |
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| QP12081-10ug | EnQuireBio | 10ug | EUR 155 |
Recombinant Influenza H3N2 Brisbane Protein, Untagged, Insect-1mg |
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| QP12081-1mg | EnQuireBio | 1mg | EUR 2059 |
Recombinant Influenza H3N2 Brisbane Protein, Untagged, Insect-50ug |
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| QP12081-50ug | EnQuireBio | 50ug | EUR 201 |
Influenza B Strain B/Florida/07/04 Antigen |
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| 11-575 | ProSci | 0.1 mg | EUR 527 |
|
Description: Influenza B (B/Florida/07/04), Influenza B Virus, Strain B/Florida/07/04 |
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Anti-07/02/07 |
|||
| STJ501223 | St John's Laboratory | 100 µg | EUR 476 |
NATtrol Influenza A H3 Virus Stock (Qualitative) (1 mL) |
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| NATFLUAH3-ST | Zeptometrix | 1 mL | EUR 1137.84 |
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
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IAV H3N2 Hemagglutinin (aa 17-530) (A/Brisbane/10/2007) [His] |
|||
| DAG2224 | Creative Diagnostics | 100 µg | EUR 913 |
IAV H1N1 nucleoprotein (aa 1-486) (A/Brisbane/10/2007) [His] |
|||
| DAG2328 | Creative Diagnostics | 100 µg | EUR 724 |