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DNA from archived dried blood spot
Optimization of extraction of genomic DNA from archived dried blood spot (DBS): potential software in epidemiological analysis & bio banking.
Background: Restricted infrastructure is out there to gather, retailer and transport venous blood in area epidemiological research. Dried blood spot (DBS) is a sturdy potential various pattern supply for epidemiological research & bio banking. A steady supply of genomic DNA (gDNA) is required for long run storage in bio financial institution for its downstream functions. Our goal is to optimize the strategies of gDNA extraction from saved DBS and with the goal of unveiling its utility in giant scale epidemiological research.
Strategies: The aim of this research was to extract the utmost quantity of gDNA from DBS on Whatman 903 protein saver card. gDNA was extracted by way of column (Qiagen) & magnetic bead based mostly (Invitrogen) strategies. Quantification of extracted gDNA was carried out with a spectrophotometer, fluorometer, and integrity analyzed by agarose gel electrophoresis.
End result: Giant variation was noticed in amount & purity (260/280 ratio, 1.8-2.9) of the extracted gDNA. The intact gDNA bands on the electrophoresis gel mirror the robustness of DBS for gDNA even after extended storage time. The extracted gDNA quantity 2.16 – 24 ng/µl is adequate for its PCR based mostly downstream software, however sadly it could possibly’t be used for entire genome sequencing or genotyping from extracted gDNA. Sequencing or genotyping could be achieved by after growing template copy quantity by way of entire genome amplification of extracted gDNA. The obtained outcomes create a base for future analysis to develop high-throughput analysis and extraction strategies from blood samples.
Conclusion: The above outcomes reveal, DBS could be utilized as a possible and sturdy pattern supply for bio banking in area epidemiological research.
Chicken TGFR ELISA Kit |
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| ECKT0062 | Abclonal | 96Tests | EUR 625.2 |
Anserini TGFR ELISA Kit |
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| EAT0062 | Abclonal | 96Tests | EUR 625.2 |
Guinea Pig TGFR ELISA Kit |
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| EGT0062 | Abclonal | 96Tests | EUR 625.2 |
Recombinant Human ALK-1 Protein |
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| RP00978 | Abclonal | 5 μg | EUR 163.2 |
Recombinant Human ALK-1 Protein |
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| VAng-2860Lsx-10g | Creative Biolabs | 10 µg | EUR 736.8 |
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Description: Recombinant Human ALK-1 was expressed in Sf9 Insect Cells. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein |
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| VAng-2860Lsx-1mg | Creative Biolabs | 1 mg | EUR 16605.6 |
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Description: Recombinant Human ALK-1 was expressed in Sf9 Insect Cells. (Uniprot ID: P37023) |
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Goat anti Human IgG (Alk Phos) |
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| 43C-CB0963 | Fitzgerald | 1 mg | EUR 433.2 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43C-CB0978 | Fitzgerald | 1 mg | EUR 483.6 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgM (Alk Phos) |
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| 43C-CB0999 | Fitzgerald | 1 mg | EUR 456 |
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Description: Goat anti Human IgM secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-1169 | Fitzgerald | 1 mg | EUR 223.2 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-1175 | Fitzgerald | 1 mg | EUR 268.8 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-1192 | Fitzgerald | 1 mg | EUR 315.6 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-1204 | Fitzgerald | 500 ug | EUR 355.2 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-IG012alk | Fitzgerald | 1 ml | EUR 658.8 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-IG165ALK | Fitzgerald | 1 mg | EUR 268.8 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Rabbit anti Human IgG (Alk Phos) |
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| 43C-CB0949 | Fitzgerald | 1 mg | EUR 526.8 |
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Description: Rabbit anti Human IgG secondary antibody (Alk Phos) |
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Rabbit anti Human IgG (Alk Phos) |
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| 43C-CB0956 | Fitzgerald | 1 mg | EUR 450 |
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Description: Rabbit anti Human IgG secondary antibody (Alk Phos) |
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Rabbit anti Human IgG (Alk Phos) |
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| 43C-CB0970 | Fitzgerald | 1 mg | EUR 450 |
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Description: Rabbit anti Human IgG secondary antibody (Alk Phos) |
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Rabbit anti Human IgM (Alk Phos) |
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| 43C-CB9106 | Fitzgerald | 1 mg | EUR 522 |
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Description: Rabbit anti Human IgM secondary antibody (Alk Phos) |
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Rabbit anti Human IgA (Alk Phos) |
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| 43C-CB9120 | Fitzgerald | 1 mg | EUR 475.2 |
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Description: Rabbit anti Human IgA secondary antibody (Alk Phos) |
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ALK-1 Polyclonal Antibody |
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| 41591-100ul | SAB | 100ul | EUR 302.4 |
ALK-1 Polyclonal Antibody |
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| 41591-50ul | SAB | 50ul | EUR 224.4 |
ALK-1 Polyclonal Antibody |
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| ABP52913-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1 |
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ALK-1 Polyclonal Antibody |
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| ABP52913-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1 |
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ALK-1 Polyclonal Antibody |
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| ABP52913-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1 |
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ALK-1 Polyclonal Antibody |
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| ABP57032-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250 |
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ALK-1 Polyclonal Antibody |
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| ABP57032-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250 |
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ALK-1 Polyclonal Antibody |
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| ABP57032-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250 |
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ALK-1 Polyclonal Antibody |
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| ES3912-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against ALK-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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ALK-1 Polyclonal Antibody |
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| ES3912-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against ALK-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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ALK-1 Polyclonal Antibody |
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| ES8031-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against ALK-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
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ALK-1 Polyclonal Antibody |
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| ES8031-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against ALK-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
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Anti-ALK-1 antibody |
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| STJ96547 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to ALK-1. |
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Anti-ALK-1 antibody |
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| STJ91563 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to ALK-1. |
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Nori® Human ALK-1 ELISA Kit |
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| GR111542 | Genorise Scientific | 96-well | EUR 461 |
Human ALK and LTK ligand 1 (ALKAL1) |
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| 1-CSB-EP757795HU | Cusabio |
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Description: Recombinant Human ALK and LTK ligand 1(ALKAL1) expressed in E.coli |
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ELISA kit for Human ALK-1/ACVRL1 |
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| EK5715 | SAB | 96 tests | EUR 663.6 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Human ALK-1/ACVRL1 in samples from serum, plasma, tissue homogenates and other biological fluids. |
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ALK-IN-1 |
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| HY-13464 | MedChemExpress | 100mg | EUR 472.8 |
Human ALK-1/ACVRL1 PicoKine ELISA Kit |
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| EK1518 | BosterBio | 96 wells | EUR 510 |
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Description: For quantitative detection of human ALK-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
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Goat anti Human IgG Fc (Alk Phos) |
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| 43-I24 | Fitzgerald | 100 ug | EUR 198 |
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Description: Goat anti Human IgG Fc secondary antibody (Alk Phos) |
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ALK inhibitor 1 |
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| HY-15357 | MedChemExpress | 50mg | EUR 1712.4 |
Goat anti Human Kappa Chain (Alk Phos) |
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| 43C-CB9127 | Fitzgerald | 1 mg | EUR 500.4 |
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Description: Goat anti Human Kappa Chain secondary antibody (Alk Phos) |
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Goat anti Human kappa chain (Alk Phos) |
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| 43R-1257 | Fitzgerald | 500 ug | EUR 482.4 |
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Description: Goat anti Human kappa chain secondary antibody (Alk Phos) |
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Goat anti Human Lambda Chain (Alk Phos) |
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| 43C-CB9134 | Fitzgerald | 1 mg | EUR 500.4 |
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Description: Goat anti Human Lambda Chain secondary antibody (Alk Phos) |
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ALK-1 Polyclonal Conjugated Antibody |
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| C41591 | SAB | 100ul | EUR 476.4 |
Goat anti Human IgG (H + L) (Alk Phos) |
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| 43C-CB0921 | Fitzgerald | 1 mg | EUR 205.2 |
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Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG (H + L) (Alk Phos) |
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| 43C-CB0928 | Fitzgerald | 1 mg | EUR 417.6 |
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Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG (H + L) (Alk Phos) |
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| 43C-CB0935 | Fitzgerald | 1 mg | EUR 543.6 |
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Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG (H + L) (Alk Phos) |
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| 43R-1145 | Fitzgerald | 1 mg | EUR 268.8 |
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Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG (H + L) (Alk Phos) |
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| 43R-1157 | Fitzgerald | 500 ug | EUR 268.8 |
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Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG (H + L) (Alk Phos) |
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| 43R-1163 | Fitzgerald | 500 ug | EUR 315.6 |
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Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Recombinant Human ALK-1 Protein (aa 22-503) |
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| VAng-2854Lsx-100g | Creative Biolabs | 100 µg | EUR 8440.8 |
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Description: Recombinant Human ALK-1 was expressed in cell free expression systems. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein (aa 22-503) |
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| VAng-2854Lsx-50g | Creative Biolabs | 50 µg | EUR 5454 |
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Description: Recombinant Human ALK-1 was expressed in cell free expression systems. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein (aa 22-118) |
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| VAng-2856Lsx-1mg | Creative Biolabs | 1 mg | EUR 3393.6 |
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Description: Recombinant Human ALK-1 was expressed in E. coli. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein (aa 22-118) |
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| VAng-2856Lsx-200g | Creative Biolabs | 200 µg | EUR 2205.6 |
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Description: Recombinant Human ALK-1 was expressed in E. coli. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein (aa 22-118) |
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| VAng-2856Lsx-500g | Creative Biolabs | 500 µg | EUR 2419.2 |
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Description: Recombinant Human ALK-1 was expressed in E. coli. (Uniprot ID: P37023) |
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ALK-1 Recombinant Protein |
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| 92-565 | ProSci | 0.05 mg | EUR 374.1 |
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Description: Activin Receptor-Like Kinase 1 (ALK-1) is a type I cell-surface receptor for the TGF- beta superfamily of ligands, which mediates signaling of BMP9 (bone morphogenetic protein) and BMP10. ALK1 signaling is necessary for angiogenesis during embryogenesis, wound healing, and tumor growth. ALK-1 has a high degree of similarity in serine-threonine kinase subdomains, a glycine and serine rich region preceding the kinase-domain, and a C-terminal tail with other activin receptor-like kinase proteins. ALK-1 is mainly expressed in endothelial cells regulating proliferation and migration in vitro and angiogenesis in vivo. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting ALK-1 plays a critical role for in the control of blood vessel development or repair. |
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Anti-ALK Antibody Clone ALK/1503, Unconjugated-20ug |
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| 238-MSM3-P0 | EnQuireBio | 20ug | EUR 279.6 |
Rabbit anti Human IgG (H + L) (Alk Phos) |
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| 43C-CB0914 | Fitzgerald | 1 mg | EUR 478.8 |
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Description: Rabbit anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG + IgA + IgM (Alk Phos) |
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| 43R-1245 | Fitzgerald | 1 mg | EUR 205.2 |
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Description: Goat anti Human IgG + IgA + IgM secondary antibody (Alk Phos) |
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Goat anti Human IgG + IgA + IgM (Alk Phos) |
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| 43R-1251 | Fitzgerald | 500 ug | EUR 268.8 |
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Description: Goat anti Human IgG + IgA + IgM secondary antibody (Alk Phos) |
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Rabbit anti Human IgG (H + L) (Alk Phos) |
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| 43R-1269 | Fitzgerald | 1 mg | EUR 295.2 |
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Description: Rabbit anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Donkey anti Human IgG (H + L) (Alk Phos) |
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| 43R-ID001alk | Fitzgerald | 500 ul | EUR 390 |
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Description: Donkey anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Rabbit anti Human IgG (H + L) (Alk Phos) |
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| 43-I23 | Fitzgerald | 1 mg | EUR 435.6 |
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Description: Rabbit anti Human IgG (H + L) secondary antibody (Alk Phos) |
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ALK sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K0074502 | ABM | 1.0 ug DNA | EUR 184.8 |
Anti-ALK Antibody Clone ALK/1503, Unconjugated-100ug |
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| 238-MSM3-P1 | EnQuireBio | 100ug | EUR 513.6 |
Goat anti Human IgM (mu chain) (Alk Phos) |
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| 43R-1227 | Fitzgerald | 1 mg | EUR 274.8 |
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Description: Goat anti Human IgM (mu chain) secondary antibody (Alk Phos) |
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Goat anti Human IgM (mu chain) (Alk Phos) |
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| 43R-1233 | Fitzgerald | 500 ug | EUR 456 |
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Description: Goat anti Human IgM (mu chain) secondary antibody (Alk Phos) |
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Goat anti Human IgM (mu chain) (Alk Phos) |
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| 43R-1239 | Fitzgerald | 500 ug | EUR 456 |
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Description: Goat anti Human IgM (mu chain) secondary antibody (Alk Phos) |
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Goat anti Human IgM (mu chain) (Alk Phos) |
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| 43-I34 | Fitzgerald | 1 mg | EUR 232.8 |
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Description: Goat anti Human IgM (mu chain) secondary antibody (Alk Phos) |
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Goat anti Human IgA (alpha chain) (Alk Phos) |
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| 43R-1119 | Fitzgerald | 500 ug | EUR 355.2 |
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Description: Goat anti Human IgA (alpha chain) secondary antibody (Alk Phos) |
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Goat anti Human IgG (gamma chain) (Alk Phos) |
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| 43R-1659 | Fitzgerald | 1 mg | EUR 301.2 |
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Description: Goat anti Human IgG (gamma chain) secondary antibody (Alkaline Phosphatase) |
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Goat anti Human IgA (alpha chain) (Alk Phos) |
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| 43-I10 | Fitzgerald | 1 mg | EUR 355.2 |
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Description: Goat anti Human IgA (alpha chain) secondary antibody (Alk Phos) |
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Goat anti Human IgE (epsilon chain) (Alk Phos) |
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| 43R-1129 | Fitzgerald | 1 mg | EUR 456 |
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Description: Goat anti Human IgE (epsilon chain) secondary antibody (Alk Phos) |
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Goat anti Human IgE (epsilon chain) (Alk Phos) |
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| 43R-1134 | Fitzgerald | 500 ug | EUR 456 |
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Description: Goat anti Human IgE (epsilon chain) secondary antibody (Alk Phos) |
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Goat Anti Human Ige Polyclonal Antibody,Alk. Phos. |
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| CPBT-65486GH | Creative Diagnostics | 0.5 mg | EUR 696 |
Recombinant Human ALK-1 Protein (aa 22-118) [Fc] |
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| VAng-2859Lsx-50g | Creative Biolabs | 50 µg | EUR 621.6 |
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Description: Recombinant Human ALK-1 was expressed in HEK 293 cells. (Uniprot ID: P37023) |
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ALK 1 Recombinant Protein |
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| 96-022 | ProSci | 0.1 mg | EUR 619.8 |
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Description: Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ALK1 gene. ALK1 is a receptor in the TGF beta signaling pathway. ALK1 protein is a receptor in the TGF beta signaling pathway. It plays an important role in vascular development, remodeling, and pathologic angiogenesis, play a role in stabilizing angiogenic vessels and contribute to resistance to anti-VEGF therapies, ALK1 blockade may represent an effective therapeutic opportunity complementary to the current antiangiogenic modalities in the clinic. Recently, researcher found that, ALK1-Fc inhibited BMP9-mediated Id-1 expression in human umbilical vein endothelial cells and inhibited cord formation by these cells on a Matrigel substrate, in a chick chorioallantoic membrane assay, ALK1-Fc reduced vascular endothelial growth factor-, fibroblast growth factor-, and BMP10-mediated vessel formation, and ALK1-Fc treatment reduced tumor burden in mice receiving orthotopic grafts of MCF7 mammary adenocarcinoma cells. |
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Recombinant Human Activin Receptor-Like Kinase 1/ALK-1(C-Fc) |
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| C422-10ug | Novoprotein | 10ug | EUR 96 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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Recombinant Human Activin Receptor-Like Kinase 1/ALK-1(C-Fc) |
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| C422-1mg | Novoprotein | 1mg | EUR 1216.8 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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Recombinant Human Activin Receptor-Like Kinase 1/ALK-1(C-Fc) |
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| C422-500ug | Novoprotein | 500ug | EUR 790.8 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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Recombinant Human Activin Receptor-Like Kinase 1/ALK-1(C-Fc) |
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| C422-50ug | Novoprotein | 50ug | EUR 169.2 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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anti- ALK,CD246 antibody |
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| FNab00309 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against ALK,CD246 |
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anti- ALK,CD246 antibody |
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| FNab00310 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against ALK,CD246 |
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Recombinant Human ALK-1 Protein (aa 238-479) [His] |
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| VAng-2861Lsx-1mg | Creative Biolabs | 1 mg | EUR 3952.8 |
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Description: Recombinant Human ALK-1 was expressed in E. coli. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein (aa 238-479) [His] |
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| VAng-2861Lsx-200g | Creative Biolabs | 200 µg | EUR 1429.2 |
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Description: Recombinant Human ALK-1 was expressed in E. coli. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein (aa 238-479) [His] |
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| VAng-2861Lsx-500g | Creative Biolabs | 500 µg | EUR 2700 |
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Description: Recombinant Human ALK-1 was expressed in E. coli. (Uniprot ID: P37023) |
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Human ALK Tyrosine Kinase Receptor (ALK) ELISA Kit |
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| abx250524-96tests | Abbexa | 96 tests | EUR 1064.4 |
Human ALK tyrosine kinase receptor (ALK) ELISA Kit |
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| abx515273-96tests | Abbexa | 96 tests | EUR 801.6 |
Comparability of 11 strategies for genomic DNA extraction appropriate for large-scale whole-genome genotyping and long-term DNA banking utilizing blood samples.
Over the latest years, subsequent technology sequencing and microarray applied sciences have revolutionized scientific analysis with their functions to high-throughput evaluation of organic methods. Isolation of excessive portions of pure, intact, double stranded, extremely concentrated, not contaminated genomic DNA is prerequisite for profitable and dependable giant scale genotyping evaluation.
Excessive portions of pure DNA are additionally required for the creation of DNA-banks. Within the current research, eleven totally different DNA extraction procedures, together with phenol-chloroform, silica and magnetic beads based mostly extractions, have been examined to determine their relative effectiveness for extracting DNA from ovine blood samples.
The standard and amount of the differentially extracted DNA was subsequently assessed by spectrophotometric measurements, Qubit measurements, real-time PCR amplifications and gel electrophoresis.
Processing time, depth of labor and value for every methodology have been additionally evaluated. Outcomes revealed vital variations among the many eleven procedures and solely 4 of the strategies yielded passable outputs.
These 4 strategies, comprising three modified silica based mostly business kits (Modified Blood, Modified Tissue, Modified Dx kits) and an in-house developed magnetic beads based mostly protocol, have been most acceptable for extracting prime quality and amount DNA appropriate for large-scale microarray genotyping and in addition for long-term DNA storage as demonstrated by their profitable software to 600 people.
DNA barcoding for conservation, seed banking and ecological restoration of Acacia within the Midwest of Western Australia.
We used DNA barcoding to deal with an essential conservation concern within the Midwest of Western Australia, engaged on Australia’s largest genus of flowering plant.
We examined whether or not or not at the moment advisable plant DNA barcoding areas (matK and rbcL) have been capable of discriminate Acacia taxa of various phylogenetic distances, and finally determine an ambiguously labelled seed assortment from a mine-site restoration venture.
Though matK efficiently recognized the unknown seed because the uncommon and conservation precedence listed A. karina, and was capable of resolve six of the eleven research species, this area was tough to amplify and sequence.
In distinction, rbcL was simple to get well and align, however couldn’t decide the origin of the seed and solely resolved three of the 11 species. Different chloroplast areas (rpl32-trnL, psbA-trnH, trnL-F and trnK) had combined success resolving the studied taxa. Usually, species have been higher resolved in multilocus knowledge units in comparison with single-locus knowledge units.
We advocate utilizing the formal barcoding areas supplemented with knowledge from different plastid areas, significantly rpl32-trnL, for barcoding in Acacia. Our research demonstrates the novel use of DNA barcoding for seed identification and illustrates the sensible potential of DNA barcoding for the rising self-discipline of restoration ecology.
Influenza A H1N1pdm (Brisbane/2/18) Culture Fluid (Heat Inactivated) (1 mL) |
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| 0810585CFHI | Zeptometrix | 1 mL | EUR 422 |
Recombinant Influenza H3N2 Brisbane Protein, Untagged, Insect-10ug |
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| QP12081-10ug | EnQuireBio | 10ug | EUR 186 |
Recombinant Influenza H3N2 Brisbane Protein, Untagged, Insect-1mg |
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| QP12081-1mg | EnQuireBio | 1mg | EUR 2470.8 |
Recombinant Influenza H3N2 Brisbane Protein, Untagged, Insect-50ug |
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| QP12081-50ug | EnQuireBio | 50ug | EUR 241.2 |
Recombinant (HEK) purified Influenza A nucleoprotein (H1N1-NP/1-486aa) protein (A/Brisbane/59/2007, >95%, His-tag) |
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| H1NP26-R-25 | Alpha Diagnostics | 25 ug | EUR 416.4 |
Recombinant (HEK) purified Influenza B HA1 protein (B/Brisbane/60/2008)(aa.16-363, >95%, his-tag) |
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| HA1B012-R-10 | Alpha Diagnostics | 10 ug | EUR 343.2 |
Recombinant (HEK) purified Influenza B HA1 protein (B/Brisbane/60/2008)(aa.16-363, >95%, his-tag) |
|||
| HA1B012-R-100 | Alpha Diagnostics | 100 ug | EUR 927.6 |
Recombinant (HEK) purified Influenza B HA protein (B/Brisbane/60/2008, ?TM, 16-548aa, >95%, his-tag) |
|||
| HA1B013-R-10 | Alpha Diagnostics | 10 ug | EUR 343.2 |
Recombinant (HEK) purified Influenza B HA protein (B/Brisbane/60/2008, ?TM, 16-548aa, >95%, his-tag) |
|||
| HA1B013-R-100 | Alpha Diagnostics | 100 ug | EUR 927.6 |
IAV H3N2 nucleoprotein (A/Brisbane/10/2007) [His] |
|||
| DAG2329 | Creative Diagnostics | 100 µg | EUR 868.8 |
IAV H3N2 (aa 18-530) (A/Brisbane/10/2007) [His] |
|||
| DAG1693 | Creative Diagnostics | 50 ug | EUR 2017.2 |
IAV H3N2 non-structural 1 (A/Brisbane/10/2007 ) [His] |
|||
| DAG2335 | Creative Diagnostics | 100 µg | EUR 868.8 |
IAV H3N2 Hemagglutinin (aa 17-530) (A/Brisbane/10/2007) [His] |
|||
| DAG2224 | Creative Diagnostics | 100 µg | EUR 1095.6 |
IAV H1N1 nucleoprotein (aa 1-486) (A/Brisbane/10/2007) [His] |
|||
| DAG2328 | Creative Diagnostics | 100 µg | EUR 868.8 |
Mouse Monoclonal Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG |
|||
| H3N21-M | Alpha Diagnostics | 100 ul | EUR 578.4 |
Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure |
|||
| H3N23-A | Alpha Diagnostics | 100 ul | EUR 534 |
IAV H3N2 Hemagglutinin 1 (aa 17-345) (A/Brisbane/10/2007) [His] |
|||
| DAG2282 | Creative Diagnostics | 100 µg | EUR 1095.6 |
IAV H1N1 (aa 18-530) (A/Brisbane/59/2007) [His] |
|||
| DAG1688 | Creative Diagnostics | 50 ug | EUR 2017.2 |
Influenza A H3 Antibody |
|||
| abx021977-1mg | Abbexa | 1 mg | EUR 1053.6 |
Influenza A H3 Antibody |
|||
| abx021978-1mg | Abbexa | 1 mg | EUR 1053.6 |
IAV H1N1 Hemagglutin (aa 51-301) (A/Brisbane/59/2007) [His] |
|||
| DAG2202 | Creative Diagnostics | 100 µg | EUR 1095.6 |
IAV H1N1 Hemagglutin (aa 18-530) (A/Brisbane/59/2007) [His] |
|||
| DAG2220 | Creative Diagnostics | 100 µg | EUR 1095.6 |
Recombinant IAV H3N2 Hemagglutinin / HA Protein (aa 1-530) [His] (A/Brisbane/10/2007) |
|||
| VAng-Wyb7007-100g | Creative Biolabs | 100 µg | EUR 2089.2 |
|
Description: Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin / HA, recombinant protein. |
|||
Recombinant IAV H3N2 Hemagglutinin / HA Protein (aa 1-530) [His] (A/Brisbane/10/2007) |
|||
| VAng-Wyb7007-50g | Creative Biolabs | 50 µg | EUR 1260 |
|
Description: Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin / HA, recombinant protein. |
|||
IAV H1N1 Hemagglutin 1 (aa 18-343) (A/Brisbane/59/2007) [His] |
|||
| DAG1775 | Creative Diagnostics | 50 ug | EUR 2017.2 |
IAV H1N1 Hemagglutin 1 (aa 18-343) (A/Brisbane/59/2007) [His] |
|||
| DAG2279 | Creative Diagnostics | 100 µg | EUR 1095.6 |
IAV H3N2 Hemagglutinin 1 (aa 17-346) (A/Brisbane/299/2011 ) [His] |
|||
| DAG2246 | Creative Diagnostics | 100 µg | EUR 1095.6 |
Recombinant IAV H1N1 Protein (aa 18-530) (A/Brisbane/59/2007) [His] |
|||
| VAng-Lsx0333-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: IAV H1N1 (aa 18-530) (A/Brisbane/59/2007) [His], recombinant protein from HEK 293 cells. |
|||
Recombinant IAV H3N2 Hemagglutinin HA1 Subunit Protein (aa 1-345) [His] (A/Brisbane/10/2007) |
|||
| VAng-Wyb7063-100g | Creative Biolabs | 100 µg | EUR 2089.2 |
|
Description: Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin HA1 Subunit, recombinant protein. |
|||
Recombinant IAV H3N2 Hemagglutinin HA1 Subunit Protein (aa 1-345) [His] (A/Brisbane/10/2007) |
|||
| VAng-Wyb7063-10g | Creative Biolabs | 10 µg | EUR 687.6 |
|
Description: Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin HA1 Subunit, recombinant protein. |
|||
Recombinant IAV H3N2 Hemagglutinin HA1 Subunit Protein (aa 1-345) [His] (A/Brisbane/10/2007) |
|||
| VAng-Wyb7063-50g | Creative Biolabs | 50 µg | EUR 1260 |
|
Description: Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin HA1 Subunit, recombinant protein. |
|||
Influenza A virus H3 PCR kit |
|||
| PCR-H649-48R | Bioingentech | 50T | EUR 987.6 |
Influenza A virus H3 PCR kit |
|||
| PCR-H649-96R | Bioingentech | 100T | EUR 1335.6 |
Influenza A Virus H3 Antigen Antibody |
|||
| abx415738-01mg | Abbexa | 0.1 mg | EUR 861.6 |
Influenza A virus H3 RT PCR kit |
|||
| RTq-H649-100R | Bioingentech | 100T | EUR 1573.2 |
Influenza A virus H3 RT PCR kit |
|||
| RTq-H649-150R | Bioingentech | 150T | EUR 2144.4 |
Influenza A virus H3 RT PCR kit |
|||
| RTq-H649-50R | Bioingentech | 50T | EUR 1155.6 |
IBV Hemagglutinin (aa 16-548) (B/Brisbane/60/2008) [His] |
|||
| DAG2215 | Creative Diagnostics | 100 µg | EUR 1095.6 |
IBV Nucleoprotein (aa 1-560) (B/Brisbane/60/2008) [His] |
|||
| DAG2319 | Creative Diagnostics | 100 µg | EUR 868.8 |
Influenza B Virus Florida 07/04 |
|||
| 7-07918 | CHI Scientific | 10µg | Ask for price |
Influenza B Virus Florida 07/04 |
|||
| 7-07919 | CHI Scientific | 50µg | Ask for price |
Influenza B Virus Florida 07/04 |
|||
| 7-07920 | CHI Scientific | 1mg | Ask for price |
IBV Matrix Proteins 1 (aa 1-252) (B/Brisbane/60/2008) [His] |
|||
| DAG2304 | Creative Diagnostics | 100 µg | EUR 868.8 |
IBV Matrix Proteins 1 (aa 1-248) (B/Brisbane/60/2008) [His] |
|||
| DAG2305 | Creative Diagnostics | 100 µg | EUR 868.8 |
Recombinant IAV H1N1 Hemagglutinin / HA Protein (aa 1-528) [His] (A/Brisbane/59/2007) |
|||
| VAng-Wyb6941-100g | Creative Biolabs | 100 µg | EUR 2092.8 |
|
Description: Influenza A H1N1 (A/Brisbane/59/2007) Hemagglutinin / HA, recombinant protein. |
|||
Recombinant IAV H1N1 Hemagglutinin / HA Protein (aa 1-528) [His] (A/Brisbane/59/2007) |
|||
| VAng-Wyb6941-10g | Creative Biolabs | 10 µg | EUR 687.6 |
|
Description: Influenza A H1N1 (A/Brisbane/59/2007) Hemagglutinin / HA, recombinant protein. |
|||
Recombinant IAV H1N1 Hemagglutinin / HA Protein (aa 1-528) [His] (A/Brisbane/59/2007) |
|||
| VAng-Wyb6941-50g | Creative Biolabs | 50 µg | EUR 1260 |
|
Description: Influenza A H1N1 (A/Brisbane/59/2007) Hemagglutinin / HA, recombinant protein. |
|||
Influenza A virus H3 One-Step PCR kit |
|||
| Oneq-H649-100R | Bioingentech | 100T | EUR 1932 |
Influenza A virus H3 One-Step PCR kit |
|||
| Oneq-H649-150R | Bioingentech | 150T | EUR 2646 |
Influenza A virus H3 One-Step PCR kit |
|||
| Oneq-H649-50R | Bioingentech | 50T | EUR 1410 |
Influenza B (B / Florida / 07 / 04) Protein |
|||
| abx060630-1mg | Abbexa | 1 mg | EUR 2247.6 |
Influenza B Virus Florida 07 / 04 Protein |
|||
| 20-abx260511 | Abbexa |
|
|
Recombinant IBV Hemagglutinin Protein (aa 16-548) (B/Brisbane/60/2008) [His] |
|||
| VAng-Lsx0372-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: IBV Hemagglutinin (aa 16-548) (B/Brisbane/60/2008) [His], recombinant protein from HEK 293 cells. |
|||
Influenza A protein |
|||
| 30-1303 | Fitzgerald | 1 mg | EUR 289.2 |
|
Description: Purified native Influenza A protein |
|||
Influenza A protein |
|||
| 30-1304 | Fitzgerald | 1 mg | EUR 354 |
|
Description: Purified native Influenza A protein |
|||
Influenza A protein |
|||
| 30-1305 | Fitzgerald | 1 mg | EUR 354 |
|
Description: Purified native Influenza A protein |
|||
Influenza A protein |
|||
| 30-1306 | Fitzgerald | 1 ml | EUR 354 |
|
Description: Purified native Influenza A protein |
|||
Influenza A protein |
|||
| 30-AI50 | Fitzgerald | 1 ml | EUR 314.4 |
|
Description: Purified Influenza A protein (H1N1 strain) |
|||
Influenza A protein |
|||
| 30-AI60 | Fitzgerald | 1 ml | EUR 314.4 |
|
Description: Purified Influenza A protein (H3N2 strain) |
|||
Influenza A protein |
|||
| 30-1002 | Fitzgerald | 100 ug | EUR 418.8 |
|
Description: Recombinant Influenza A protein (NP antigen) |
|||
Influenza A antibody |
|||
| 10-1096 | Fitzgerald | 500 ug | EUR 249.6 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1097 | Fitzgerald | 1 mg | EUR 418.8 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1098 | Fitzgerald | 1 mg | EUR 726 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1099 | Fitzgerald | 1 mg | EUR 726 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1100 | Fitzgerald | 500 ug | EUR 418.8 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1101 | Fitzgerald | 1 mg | EUR 726 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1102 | Fitzgerald | 500 ug | EUR 418.8 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1103 | Fitzgerald | 500 ug | EUR 418.8 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1241 | Fitzgerald | 500 ug | EUR 418.8 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-1243 | Fitzgerald | 500 ug | EUR 418.8 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-7866 | Fitzgerald | 1 mg | EUR 483.6 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-7867 | Fitzgerald | 1 mg | EUR 483.6 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-I50A | Fitzgerald | 1 mg | EUR 603.6 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-I50B | Fitzgerald | 1 mg | EUR 470.4 |
|
Description: Mouse monoclonal Influenza A antibody |
|||
Influenza A antibody |
|||
| 10-I50C | Fitzgerald | 1 mg | EUR 470.4 |
|
Description: Mouse monoclonal Influenza A antibody |
|||