Proteomic evaluation revealed widespread, distinctive and systemic signatures in gender-dependent hepatocarcinogenesis
Hepatocellular carcinoma (HCC) is the commonest liver most cancers and is very malignant. Male prevalence and frequent activation of the Ras signaling pathway are distinct traits of HCC. Nonetheless, the underlying mechanisms stay to be elucidated.
- By exploring Hras12V transgenic mice displaying male-biased hepatocarcinogenesis, we carried out a high-throughput comparative proteomic evaluation primarily based on tandem-mass-tag (TMT) labeling mixed with liquid chromatography-tandem mass spectrometry (LC-MS/MS) on the tissue samples obtained from HCC (T) and their paired adjoining precancerous (P) of Hras12V transgenic female and male mice (Ras-Tg) and regular liver (W) of wild-type female and male mice (Non-Tg). The additional validation and investigation had been carried out utilizing quantitative real-time PCR and western blot. Completely, 5193 proteins had been quantified, originating from 5733 recognized proteins.
- Lastly, 1344 differentially expressed proteins (DEPs) (quantified in all examined samples; |ratios| ≥ 1.5, p < 0.05) had been chosen for additional evaluation. Comparability inside W, P, and T of men and women indicated that the variety of DEPs in males was a lot greater than that in females.
- Bioinformatics analyses confirmed the widespread and distinctive cluster-enriched gadgets between sexes, indicating the widespread and gender-disparate pathways in direction of HCC. Expression change sample evaluation revealed HCC optimistic/negative-correlated and ras oncogene optimistic/negative-correlated DEPs and pathways. As well as, it confirmed that the ras oncogene progressively and considerably lowered the responses to intercourse hormones from hepatocytes to hepatoma cells and subsequently shrunk the gender disparity between men and women, which can contribute to the reason for the lack of HCC scientific responses to the therapeutic approaches focusing on intercourse hormone pathways.
- Moreover, gender disparity within the expression ranges of key enzymes concerned in retinol metabolism and terpenoid spine/steroid biosynthesis pathways might contribute to male prevalence in hepatocarcinogenesis. Additional, the biomarkers, SAA2, Orm2, and Serpina1e, could also be intercourse variations. In conclusion, widespread and distinctive DEPs and pathways towards HCC initiated by ras oncogene from sexually dimorphic hepatocytes present beneficial and novel insights into scientific investigation and apply.
MagSi-proteomics C4 |
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| MD02014 | AMSBIO | 10 mL | EUR 1332 |
MagSi-proteomics C8 |
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| MD02015 | AMSBIO | 10 mL | EUR 1332 |
MagSi-proteomics C4 |
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| MD03014 | AMSBIO | 100 mL | EUR 7260 |
MagSi-proteomics C8 |
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| MD03015 | AMSBIO | 100 mL | EUR 7260 |
AlbuVoid™ LC-MS On-Bead For Serum Proteomics |
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| AVB-MS05 | Biotech Support Group | 5 preps | EUR 454.8 |
|
Description: Albumin Removal Kit |
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AlbuVoid™ LC-MS On-Bead For Serum Proteomics |
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| AVB-MS10 | Biotech Support Group | 10 preps | EUR 625.2 |
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Description: Albumin Removal Kit |
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HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics |
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| HVB-MS05 | Biotech Support Group | 5 preps | EUR 454.8 |
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Description: Hemoglobin Removal Kit |
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HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics |
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| HVB-MS10 | Biotech Support Group | 10 preps | EUR 625.2 |
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Description: Hemoglobin Removal Kit |
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MagSi-WCX |
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| MD01023 | AMSBIO | 2 mL | EUR 435.6 |
MagSi-WAX |
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| MD01025 | AMSBIO | 2 mL | EUR 435.6 |
MagSi-WCX |
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| MD02023 | AMSBIO | 10 mL | EUR 1488 |
MagSi-WAX |
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| MD02025 | AMSBIO | 10 mL | EUR 1488 |
MagSi-WCX |
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| MD03023 | AMSBIO | 100 mL | EUR 7260 |
MagSi-WAX |
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| MD03025 | AMSBIO | 100 mL | EUR 7260 |
MagSi-cfDNA |
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| MDKT00220096 | AMSBIO | 96 preps | EUR 1056 |
MagSi-S 1.0 |
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| MD01003 | AMSBIO | 2 mL | EUR 58.8 |
MagSi-S 1.0 |
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| MD03003 | AMSBIO | 10 mL | EUR 163.2 |
MagSi-S 1.0 |
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| MD04003 | AMSBIO | 100 mL | EUR 1296 |
MagSi-S 600 |
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| MD16003 | AMSBIO | 2 mL | EUR 61.2 |
MagSi-S 600 |
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| MD18003 | AMSBIO | 10 mL | EUR 170.4 |
MagSi-S 600 |
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| MD19003 | AMSBIO | 100 mL | EUR 1500 |
MagSi-S 3.0 |
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| MD41003 | AMSBIO | 2 mL | EUR 58.8 |
MagSi-S 3.0 |
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| MD43003 | AMSBIO | 10 mL | EUR 163.2 |
MagSi-S 3.0 |
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| MD44003 | AMSBIO | 100 mL | EUR 1296 |
MagSi-STA 1.0 |
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| MD01001 | AMSBIO | 2 mL | EUR 426 |
MagSi-DNA 600 |
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| MD01016 | AMSBIO | 2 mL | EUR 87.6 |
MagSi-DNA 3.0 |
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| MD01022 | AMSBIO | 2 mL | EUR 87.6 |
MagSi-DNA mf |
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| MD0200010002 | AMSBIO | 2 mL | EUR 114 |
MagSi-DNA mf |
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| MD0200010010 | AMSBIO | 10 mL | EUR 441.6 |
MagSi-DNA mf |
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| MD0200010100 | AMSBIO | 100 mL | EUR 3618 |
MagSi-DNA 600 |
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| MD02016 | AMSBIO | 10 mL | EUR 339.6 |
MagSi-STA 1.0 |
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| MD03001 | AMSBIO | 10 mL | EUR 1434 |
MagSi-DNA 600 |
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| MD03016 | AMSBIO | 100 mL | EUR 2796 |
MagSi-DNA 3.0 |
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| MD03022 | AMSBIO | 10 mL | EUR 339.6 |
MagSi-STA 1.0 |
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| MD04001 | AMSBIO | 100 mL | EUR 7158 |
MagSi-DNA 3.0 |
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| MD04022 | AMSBIO | 100 mL | EUR 2796 |
MagSi-STA 600 |
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| MD16001 | AMSBIO | 2 mL | EUR 470.4 |
MagSi-STA 600 |
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| MD18001 | AMSBIO | 10 mL | EUR 1572 |
MagSi-STA 600 |
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| MD19001 | AMSBIO | 100 mL | EUR 9060 |
MagSi-DT Removal* |
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| MDKT00040008 | AMSBIO | 8 mL | EUR 181.2 |
MagSi-DT Removal* |
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| MDKT00040050 | AMSBIO | 50 mL | EUR 1092 |
MagSi-DT Removal* |
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| MDKT00040500 | AMSBIO | 500 mL | EUR 6426 |
MagSi-DNA Animal |
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| MDKT00150096 | AMSBIO | 96 preps | EUR 259.2 |
MagSi-DNA Animal |
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| MDKT00150960 | AMSBIO | 10 x 96 preps | EUR 2076 |
MagSi-protein A 1.0 |
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| MD01011 | AMSBIO | 1 mL | EUR 152.4 |
MagSi-protein G 1.0 |
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| MD01012 | AMSBIO | 1 mL | EUR 152.4 |
MagSi-protein A 1.0 |
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| MD02011 | AMSBIO | 5 mL | EUR 607.2 |
MagSi-protein G 1.0 |
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| MD02012 | AMSBIO | 5 mL | EUR 607.2 |
MagSi-protein A 600 |
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| MD10011 | AMSBIO | 1 mL | EUR 181.2 |
MagSi-protein G 600 |
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| MD10012 | AMSBIO | 1 mL | EUR 181.2 |
MagSi-protein A 600 |
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| MD11011 | AMSBIO | 5 mL | EUR 722.4 |
MagSi-protein G 600 |
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| MD11012 | AMSBIO | 5 mL | EUR 722.4 |
MagSi-protein A 3.0 |
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| MD41011 | AMSBIO | 1 mL | EUR 152.4 |
MagSi-protein G 3.0 |
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| MD41012 | AMSBIO | 1 mL | EUR 152.4 |
MagSi-protein A 3.0 |
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| MD42011 | AMSBIO | 5 mL | EUR 607.2 |
MagSi-protein G 3.0 |
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| MD42012 | AMSBIO | 5 mL | EUR 607.2 |
MagSi-DX Pathogen |
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| MDDX0001005K | AMSBIO | 5K preps | EUR 11880 |
MagSi-DX Pathogen |
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| MDDX00010096 | AMSBIO | 96 preps | EUR 300 |
MagSi-DX Pathogen |
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| MDDX0001025K | AMSBIO | 25K preps | EUR 58800 |
MagSi-DX Pathogen |
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| MDDX00010960 | AMSBIO | 10x96 preps | EUR 2398.8 |
MagSi-DNA allround |
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| MD01018 | AMSBIO | 2 mL | EUR 87.6 |
MagSi-DNA allround |
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| MD02018 | AMSBIO | 10 mL | EUR 339.6 |
MagSi-DNA allround |
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| MD03018 | AMSBIO | 100 mL | EUR 2796 |
MagSi-NGSPREP Plus* |
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| MDKT00010005 | AMSBIO | 5 mL | EUR 76.8 |
MagSi-NGSPREP Plus* |
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| MDKT00010075 | AMSBIO | 75 mL | EUR 714 |
MagSi-NGSPREP Plus* |
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| MDKT00010500 | AMSBIO | 500 mL | EUR 3564 |
MagSi-NA Pathogens |
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| MDKT0021005K | AMSBIO | 5K preps | EUR 11880 |
MagSi-NA Pathogens |
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| MDKT00210096 | AMSBIO | 96 preps | EUR 300 |
MagSi-NA Pathogens |
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| MDKT0021025K | AMSBIO | 25K preps | EUR 58800 |
MagSi-NA Pathogens |
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| MDKT00210960 | AMSBIO | 10x96 preps | EUR 2398.8 |
MagSi-S SH 1.0 |
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| MD03006 | AMSBIO | 10 mL | EUR 483.6 |
MagSi-S SH 1.0 |
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| MD04006 | AMSBIO | 100 mL | EUR 1548 |
MagSi-S SH 600 |
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| MD18006 | AMSBIO | 10 mL | EUR 538.8 |
MagSi-S SH 600 |
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| MD19006 | AMSBIO | 100 mL | EUR 1854 |
MagSi-S SH 3.0 |
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| MD43006 | AMSBIO | 10 mL | EUR 483.6 |
MagSi-S SH 3.0 |
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| MD44006 | AMSBIO | 100 mL | EUR 1548 |
MagSi-S NH2 1.0 |
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| MD01005 | AMSBIO | 2 mL | EUR 73.2 |
MagSi-S NH2 1.0 |
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| MD03005 | AMSBIO | 10 mL | EUR 204 |
MagSi-S CHO 1.0 |
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| MD03007 | AMSBIO | 10 mL | EUR 483.6 |
MagSi-S NH2 1.0 |
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| MD04005 | AMSBIO | 100 mL | EUR 1638 |
MagSi-S CHO 1.0 |
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| MD04007 | AMSBIO | 100 mL | EUR 1548 |
MagSi-S NH2 600 |
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| MD16005 | AMSBIO | 2 mL | EUR 87.6 |
MagSi-S NH2 600 |
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| MD18005 | AMSBIO | 10 mL | EUR 246 |
MagSi-S CHO 600 |
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| MD18007 | AMSBIO | 10 mL | EUR 538.8 |
MagSi-S NH2 600 |
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| MD19005 | AMSBIO | 100 mL | EUR 1980 |
MagSi-S CHO 600 |
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| MD19007 | AMSBIO | 100 mL | EUR 1854 |
MagSi-S NH2 3.0 |
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| MD41005 | AMSBIO | 2 mL | EUR 73.2 |
Identification and Validation of Stage-Related PBMC Biomarkers in Breast Most cancers Utilizing MS-Primarily based Proteomics
Background: It’s well-described that the transcriptome of peripheral blood mononuclear cells (PBMCs) might be altered within the context of many malignancies to permit them keep away from the efficient immune response, which ends up in most cancers invasiveness. Right here, we used an MS-based technique to find biomarkers within the PBMCs of breast most cancers (BC) sufferers and validated them at completely different phases of BC.
Strategies: PBMCs had been remoted from the breast most cancers sufferers and had been cultured alone or co-cultured with breast most cancers cell traces. The function of PBMC within the invasion property of breast most cancers cells was explored. NF-kB exercise was additionally measured within the co-cultured breast most cancers cells. Identification of protein profiles within the secretome and proteome of the co-cultured PBMCs was carried out utilizing SWATH mass spectrometry. Pathway enrichment and gene ontology analyses had been carried out to search for the molecular pathways correlated with the protein expression profile of PBMCs within the breast most cancers sufferers. Quantitative real-time polymerase chain response (qPCR) was carried out to validate the candidate genes within the PBMC fraction of the breast most cancers sufferers on the major and metastatic phases. In silico survival evaluation was carried out to evaluate the potential scientific biomarkers in these PBMC subtypes.
Outcomes: PBMCs might considerably enhance the invasion property of the BC cells concomitant with a lower in E-cadherin and a rise in each Vimentin and N-cadherin expression. The NF-kB exercise within the BC cells considerably elevated following co-culturing implying the function of PBMCs in EMT induction. Enrichment evaluation confirmed that the differentially expressed proteins in PBMCs are primarily related to IL-17, PI3K-Akt, and HIF-1 signaling pathway, through which a set of seven proteins together with TMSB4X, HSPA4, S100A9, SRSF6, THBS1, CUL4A, and CANX had been incessantly expressed. Lastly, in silico evaluation confirmed {that a} gene set consisting of S100A9, SRSF6, THBS1, CUL4A, and CANX had been discovered to offer an perception for the identification of metastasis in breast most cancers sufferers.
Conclusion: In conclusion, our research revealed that the protein expression profile in PBMCs is a mirrored image of the proteins expressed within the BC tissue itself; nonetheless, the abundance degree is completely different because of the stage of most cancers.
Anti-HA magnetic beads |
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| B26202 | Bimake | 5 mL | EUR 1742.4 |
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Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification. |
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Anti-HA Magnetic Beads |
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| HY-K0201 | MedChemExpress | 1 mL | EUR 334.8 |
Anti-Myc magnetic beads |
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| B26301 | Bimake | 1 mL | EUR 579.6 |
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Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification. |
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Anti-Myc magnetic beads |
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| B26302 | Bimake | 5 mL | EUR 1742.4 |
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Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification. |
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Protein A/G/L Magnetic Beads |
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| 6547-1 | Biovision | each | EUR 405.6 |
Anti-Flag magnetic beads |
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| B26101 | Bimake | 1 mL | EUR 579.6 |
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Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification. |
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Anti-Flag magnetic beads |
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| B26102 | Bimake | 5 mL | EUR 1742.4 |
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Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification. |
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Anti-Flag Magnetic Beads |
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| HY-K0207 | MedChemExpress | 1 mL | EUR 570 |
Magnetic Beads (DNA) 30 mL |
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| P920-30 | 101Bio | - | Ask for price |
Magnetic Beads (DNA) 450 mL |
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| P920-450 | 101Bio | - | Ask for price |
Magnetic Beads (DNA) 5 mL |
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| P920-5 | 101Bio | - | Ask for price |
Magnetic Beads (DNA) 60 mL |
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| P920-60 | 101Bio | - | Ask for price |
Protein A/G Magnetic Beads for IP |
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| B23201 | Bimake | 1 ml | EUR 158.4 |
|
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens. |
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Protein A/G Magnetic Beads for IP |
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| B23202 | Bimake | 5 ml | EUR 558 |
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Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens. |
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Anti-c-Myc Magnetic Beads |
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| HY-K0206 | MedChemExpress | 5 mL | EUR 1368 |
Magnetic Beads for DNA Purification |
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| M1502-5 | Biovision | 5 ml | EUR 332.4 |
FFPE Tissue DNA Extraction Kit - Magnetic Beads |
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| K5011450 | Biochain | 1 kit | EUR 237 |
Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb |
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| AE037 | Abclonal | 50 ul | EUR 211.2 |
RNA/DNA Purification Kit (Magnetic Bead) |
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| DA0623 | Daan Gene | 32 test/kit | Ask for price |
Trypsin, Recombinant, Proteomics grade |
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| P1228-1000 | Biovision | each | EUR 235.2 |
Trypsin, Recombinant, Proteomics grade |
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| P1228-10000 | Biovision | each | EUR 1468.8 |
Trypsin, Recombinant, Proteomics grade |
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| P1228-5000 | Biovision | each | EUR 738 |
Nickel NTA Magnetic Agarose Bead (5%) |
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| MagNTANi5-10 | ABTBeads | 10ml suspension | EUR 340.8 |
Nickel NTA Magnetic Agarose Bead (5%) |
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| MagNTANi5-2 | ABTBeads | 2ml suspension | EUR 118.8 |
Nickel NTA Magnetic Agarose Bead (5%) |
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| MagNTANi5-5 | ABTBeads | 5ml suspension | EUR 208.8 |
CD9 Exo-Flow capture kit (Magnetic streptavidin beads, CD9-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW100A-1 | SBI | 10 reactions | EUR 500 |
Basic Exo-Flow kit without any coupled antibodies (Uncoupled magnetic streptavidin beads, Wash and Elution Buffers, Exo-FITC stain) |
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| CSFLOWBASICA-1 | SBI | 100 reactions | EUR 853 |
CD31 Exo-Flow capture kit (Magnetic streptavidin beads, CD31-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW200A-1 | SBI | 10 reactions | EUR 500 |
CD44 Exo-Flow capture kit (Magnetic streptavidin beads, CD44-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW210A-1 | SBI | 10 reactions | EUR 500 |
CD63 Exo-Flow capture kit (Magnetic streptavidin beads, CD63-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW300A-1 | SBI | 10 reactions | EUR 500 |
CD81 Exo-Flow capture kit (Magnetic streptavidin beads, CD81-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW400A-1 | SBI | 10 reactions | EUR 500 |
Rab5b Exo-Flow capture kit (Magnetic streptavidin beads, Rab5b-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW500A-1 | SBI | 10 reactions | EUR 500 |
CD14 Exo-Flow capture kit (Magnetic streptavidin beads, CD14-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW610A-1 | SBI | 10 reactions | EUR 500 |
CD68 Exo-Flow capture kit (Magnetic streptavidin beads, CD68-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW620A-1 | SBI | 10 reactions | EUR 500 |
EpCAM Exo-Flow capture kit (Magnetic streptavidin beads, EpCAM-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW630A-1 | SBI | 10 reactions | EUR 500 |
CD73 Exo-Flow capture kit (Magnetic streptavidin beads, CD73-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW660A-1 | SBI | 10 reactions | EUR 500 |
AlbuVoid™ LC-MS On-Bead For Serum Proteomics |
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| AVB-MS05 | Biotech Support Group | 5 preps | EUR 454.8 |
|
Description: Albumin Removal Kit |
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AlbuVoid™ LC-MS On-Bead For Serum Proteomics |
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| AVB-MS10 | Biotech Support Group | 10 preps | EUR 625.2 |
|
Description: Albumin Removal Kit |
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HLA-G Exo-Flow capture kit (Magnetic streptavidin beads, HLA-G-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW600A-1 | SBI | 10 reactions | EUR 500 |
HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics |
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| HVB-MS05 | Biotech Support Group | 5 preps | EUR 454.8 |
|
Description: Hemoglobin Removal Kit |
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HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics |
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| HVB-MS10 | Biotech Support Group | 10 preps | EUR 625.2 |
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Description: Hemoglobin Removal Kit |
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Magnetic Stand |
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| abx799001-1Unit | Abbexa | 1 Unit | EUR 309.6 |
Magnetic Stand |
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| HY-K0200 | MedChemExpress | 200 μL-2 mL-15 mL | EUR 456 |
Magnetic Stirrer |
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| abx725038-1Unit | Abbexa | 1 Unit | EUR 518.4 |
Magnetic Stirrer |
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| H3760-SE | Benchmark Scientific | 1 PC | EUR 121.85 |
Protein G Magnetic Particles |
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| PGM-40-5 | Spherotech | 5 mL | EUR 346.8 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Protein G Magnetic Particles |
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| PGMS-30-5 | Spherotech | 5 mL | EUR 364.8 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Protein G Magnetic Particles |
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| PGMS-40-5 | Spherotech | 5 mL | EUR 364.8 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Protein G Magnetic Particles |
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| PGMX-10-5 | Spherotech | 5 mL | EUR 249.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Protein A Magnetic Particles |
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| PAM-40-5 | Spherotech | 5 mL | EUR 303.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Protein A Magnetic Particles |
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| PAMS-30-5 | Spherotech | 5 mL | EUR 327.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Protein A Magnetic Particles |
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| PAMS-40-5 | Spherotech | 5 mL | EUR 327.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Protein A Magnetic Particles |
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| PAMX-10-5 | Spherotech | 5 mL | EUR 249.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Magnetic Separator |
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| B23803 | Bimake | 2 mL/200 uL/15 mL | EUR 291.6 |
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Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time. |
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Magnetic Separator |
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| MagSep1-1 | ABTBeads | 1/pk | EUR 132 |
Magnetic Wand - Set |
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| M-R-1013197 | MiTeGen | 1 WAND | EUR 131 |
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Description: Magnetic Wand - Set |
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Tetraspanin Exo-Flow Combo capture kit (Magnetic streptavidin beads, CD9, CD63, and CD81-biotin capture antibodies, Wash and Elution Buffers, Exo-FITC stain) |
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| EXOFLOW150A-1 | SBI | 10 reactions | EUR 760 |
Magnetic Wand - Curved |
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| M-R-1013199 | MiTeGen | 1 WAND | EUR 74 |
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Description: Magnetic Wand - Curved |
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Magnetic Stirrer, 115V |
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| H3770-S | Benchmark Scientific | 1 each | EUR 313.4 |
Magnetic Stirrer, 230V |
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| H3770-SE | Benchmark Scientific | 1 each | EUR 313.4 |
Magnetic Wand - Straight |
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| M-R-1013198 | MiTeGen | 1 WAND | EUR 74 |
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Description: Magnetic Wand - Straight |
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GFP-nAb Magnetic 0.5ml |
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| ABP-NAB-GFPM050 | Allele Biotech | 500 µl | Ask for price |
GFP-nAb Magnetic 1ml |
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| ABP-NAB-GFPM100 | Allele Biotech | 1 ml | Ask for price |
RFP-nAb Magnetic 0.5ml |
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| ABP-NAB-RFPM050 | Allele Biotech | 500 µl | Ask for price |
RFP-nAb Magnetic 1ml |
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| ABP-NAB-RFPM100 | Allele Biotech | 1 ml | Ask for price |
Amino Magnetic Particles |
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| AMS-40-10 | Spherotech | 10 mL | EUR 286.8 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AMS-40-100 | Spherotech | 100 mL | EUR 1862.4 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AMS-40-10H | Spherotech | 10 mL | EUR 303.6 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AMX-10-10 | Spherotech | 10 mL | EUR 230.4 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AMX-10-100 | Spherotech | 100 mL | EUR 1424.4 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AMX-150-5 | Spherotech | 5 mL | EUR 444 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AMX-30-10 | Spherotech | 10 mL | EUR 230.4 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AMX-40-10 | Spherotech | 10 mL | EUR 230.4 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-10-10 | Spherotech | 10 mL | EUR 254.4 |
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Description: Amino Superparamagnetic Particles are recomended for variety of applications such as cell separation, affinity purification, DNA probe assays, magnetic particle EIA, etc. The key features are does not contain any free iron oxide, thus reducing undesirable interference, you may obtain iable and functionally active cells using using magnetic labeling after coupling with cell surface molecules. |
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Amino Magnetic Particles |
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| AM-10-100 | Spherotech | 100 mL | EUR 1618.8 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-15-10 | Spherotech | 10 mL | EUR 254.4 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-15-100 | Spherotech | 100 mL | EUR 1618.8 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-20-10 | Spherotech | 10 mL | EUR 261.6 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-20-100 | Spherotech | 100 mL | EUR 1668 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-30-10 | Spherotech | 10 mL | EUR 261.6 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-30-100 | Spherotech | 100 mL | EUR 1668 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-40-10 | Spherotech | 10 mL | EUR 261.6 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-40-100 | Spherotech | 100 mL | EUR 1668 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-60-10 | Spherotech | 10 mL | EUR 376.8 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-60-100 | Spherotech | 100 mL | EUR 2593.2 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Amino Magnetic Particles |
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| AM-80-10 | Spherotech | 10 mL | EUR 444 |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Magnetic Separation Rack |
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| M1501-1 | Biovision | 1 Rack | EUR 454.8 |
Epoxy Magnetic Particles |
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| EM-05-10 | Spherotech | 10 mL | EUR 267.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Epoxy Magnetic Particles |
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| EM-20-10 | Spherotech | 10 mL | EUR 267.6 |
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Description: Epoxy Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Epoxy Magnetic Particles |
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| EM-20-100 | Spherotech | 100 mL | EUR 1716 |
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Description: Epoxy Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Magnetic Separation Rack |
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| CM101 | Vazyme | 32 well (200 μl/well) | EUR 112 |
Magnetic Separation Rack |
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| CM103 | Vazyme | 24 well (1.5 ml/well) | EUR 112 |
GFP-nAb Magnetic 0.25ml |
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| ABP-NAB-GFPM025 | Allele Biotech | 250 µl | Ask for price |
RFP-nAb Magnetic 0.25ml |
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| ABP-NAB-RFPM025 | Allele Biotech | 250 µl | Ask for price |
Biotin Magnetic Particles |
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| TM-10-10 | Spherotech | 10 mL | EUR 254.4 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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