Proteomic analysis revealed common, unique and systemic signatures

Proteomic evaluation revealed widespread, distinctive and systemic signatures in gender-dependent hepatocarcinogenesis

Hepatocellular carcinoma (HCC) is the commonest liver most cancers and is very malignant. Male prevalence and frequent activation of the Ras signaling pathway are distinct traits of HCC. Nonetheless, the underlying mechanisms stay to be elucidated.
  • By exploring Hras12V transgenic mice displaying male-biased hepatocarcinogenesis, we carried out a high-throughput comparative proteomic evaluation primarily based on tandem-mass-tag (TMT) labeling mixed with liquid chromatography-tandem mass spectrometry (LC-MS/MS) on the tissue samples obtained from HCC (T) and their paired adjoining precancerous (P) of Hras12V transgenic female and male mice (Ras-Tg) and regular liver (W) of wild-type female and male mice (Non-Tg). The additional validation and investigation had been carried out utilizing quantitative real-time PCR and western blot. Completely, 5193 proteins had been quantified, originating from 5733 recognized proteins.
  • Lastly, 1344 differentially expressed proteins (DEPs) (quantified in all examined samples; |ratios| ≥ 1.5, p < 0.05) had been chosen for additional evaluation. Comparability inside W, P, and T of men and women indicated that the variety of DEPs in males was a lot greater than that in females.
  • Bioinformatics analyses confirmed the widespread and distinctive cluster-enriched gadgets between sexes, indicating the widespread and gender-disparate pathways in direction of HCC. Expression change sample evaluation revealed HCC optimistic/negative-correlated and ras oncogene optimistic/negative-correlated DEPs and pathways. As well as, it confirmed that the ras oncogene progressively and considerably lowered the responses to intercourse hormones from hepatocytes to hepatoma cells and subsequently shrunk the gender disparity between men and women, which can contribute to the reason for the lack of HCC scientific responses to the therapeutic approaches focusing on intercourse hormone pathways.
  • Moreover, gender disparity within the expression ranges of key enzymes concerned in retinol metabolism and terpenoid spine/steroid biosynthesis pathways might contribute to male prevalence in hepatocarcinogenesis. Additional, the biomarkers, SAA2, Orm2, and Serpina1e, could also be intercourse variations. In conclusion, widespread and distinctive DEPs and pathways towards HCC initiated by ras oncogene from sexually dimorphic hepatocytes present beneficial and novel insights into scientific investigation and apply.
postgenomeconsortium
postgenomeconsortium

ß-OG Lysis Buffer for Proteomics, pH 7.4

22050064-1 10 mL
EUR 32.55

ß-OG Lysis Buffer for Proteomics, pH 7.4

22050064-2 25 mL
EUR 63.96

ß-OG Lysis Buffer for Proteomics, pH 7.4

22050064-3 50 mL
EUR 105.49

AlbuVoid™ LC-MS On-Bead For Serum Proteomics

AVB-MS05 5 preps
EUR 454.8
Description: Albumin Removal Kit

AlbuVoid™ LC-MS On-Bead For Serum Proteomics

AVB-MS10 10 preps
EUR 585

HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics

HVB-MS05 5 preps
EUR 454.8
Description: Hemoglobin Removal Kit

HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics

HVB-MS10 10 preps
EUR 585

MagSi-proteomics C4

MD01014 2 mL
EUR 391.2

MagSi-proteomics C8

MD01015 2 mL
EUR 391.2

MagSi-proteomics C4

MD02014 10 mL
EUR 1332

MagSi-proteomics C8

MD02015 10 mL
EUR 1332

MagSi-proteomics C4

MD03014 100 mL
EUR 7260

MagSi-proteomics C8

MD03015 100 mL
EUR 7260

MagSi-WCX

MD01023 2 mL
EUR 435.6

MagSi-WAX

MD01025 2 mL
EUR 435.6

MagSi-WCX

MD02023 10 mL
EUR 1488

MagSi-WAX

MD02025 10 mL
EUR 1488

MagSi-WCX

MD03023 100 mL
EUR 7260

MagSi-WAX

MD03025 100 mL
EUR 7260

MagSi-cfDNA

MDKT00220096 96 preps
EUR 733.32

MagSi-S 1.0

MD01003 2 mL
EUR 58.8

MagSi-S 1.0

MD03003 10 mL
EUR 163.2

MagSi-S 1.0

MD04003 100 mL
EUR 1296

MagSi-S 600

MD16003 2 mL
EUR 61.2

MagSi-S 600

MD18003 10 mL
EUR 170.4

MagSi-S 600

MD19003 100 mL
EUR 1500

MagSi-S 3.0

MD41003 2 mL
EUR 58.8

MagSi-S 3.0

MD43003 10 mL
EUR 163.2

MagSi-S 3.0

MD44003 100 mL
EUR 1296

MagSi-STA 1.0

MD01001 2 mL
EUR 426

MagSi-DNA 600

MD01016 2 mL
EUR 87.6

MagSi-DNA 3.0

MD01022 2 mL
EUR 87.6

MagSi-DNA mf

MD0200010002 2 mL
EUR 114

MagSi-DNA mf

MD0200010010 10 mL
EUR 441.6

MagSi-DNA mf

MD0200010100 100 mL
EUR 3618

MagSi-DNA 600

MD02016 10 mL
EUR 339.6

MagSi-STA 1.0

MD03001 10 mL
EUR 1434

MagSi-DNA 600

MD03016 100 mL
EUR 2796

MagSi-DNA 3.0

MD03022 10 mL
EUR 339.6

MagSi-STA 1.0

MD04001 100 mL
EUR 7158

MagSi-DNA 3.0

MD04022 100 mL
EUR 2796

MagSi-STA 600

MD16001 2 mL
EUR 470.4

MagSi-STA 600

MD18001 10 mL
EUR 1572

MagSi-STA 600

MD19001 100 mL
EUR 9060

MagSi-DNA FFPE

MDKT00240096 96 preps
EUR 396.36

MagSi-DNA FFPE

MDKT00240960 10 x 96 preps
EUR 3167.64

MagSi-DNA Stool

MDKT00230096 96 preps
EUR 278.64

MagSi-DNA Stool

MDKT00230960 10 x 96 preps
EUR 2361.96

MagSi-DT Removal*

MDKT00040008 8 mL
EUR 181.2

MagSi-DT Removal*

MDKT00040050 50 mL
EUR 1092

MagSi-DT Removal*

MDKT00040500 500 mL
EUR 6426

MagSi-DNA Animal

MDKT00150096 96 preps
EUR 259.2

MagSi-DNA Animal

MDKT00150960 10 x 96 preps
EUR 2076

MagSi-protein A 1.0

MD01011 1 mL
EUR 152.4

MagSi-protein G 1.0

MD01012 1 mL
EUR 152.4

MagSi-protein A 1.0

MD02011 5 mL
EUR 607.2

MagSi-protein G 1.0

MD02012 5 mL
EUR 607.2

MagSi-protein A 600

MD10011 1 mL
EUR 181.2

MagSi-protein G 600

MD10012 1 mL
EUR 181.2

MagSi-protein A 600

MD11011 5 mL
EUR 722.4

MagSi-protein G 600

MD11012 5 mL
EUR 722.4

MagSi-protein A 3.0

MD41011 1 mL
EUR 152.4

MagSi-protein G 3.0

MD41012 1 mL
EUR 152.4

MagSi-protein A 3.0

MD42011 5 mL
EUR 607.2

MagSi-protein G 3.0

MD42012 5 mL
EUR 607.2

MagSi-DX Pathogen

MDDX0001005K 5K preps
EUR 11880

MagSi-DX Pathogen

MDDX00010096 96 preps
EUR 300

MagSi-DX Pathogen

MDDX0001025K 25K preps
EUR 58800

MagSi-DX Pathogen

MDDX00010960 10x96 preps
EUR 2398.8

MagSi-DNA allround

MD01018 2 mL
EUR 87.6

MagSi-DNA allround

MD02018 10 mL
EUR 339.6

MagSi-DNA allround

MD03018 100 mL
EUR 2796

MagSi-NGSPREP Plus*

MDKT00010005 5 mL
EUR 76.8

MagSi-NGSPREP Plus*

MDKT00010075 75 mL
EUR 714

MagSi-NGSPREP Plus*

MDKT00010500 500 mL
EUR 3564

MagSi-NA Pathogens

MDKT0021005K 5K preps
EUR 11880

MagSi-NA Pathogens

MDKT00210096 96 preps
EUR 300

MagSi-NA Pathogens

MDKT0021025K 25K preps
EUR 58800

MagSi-NA Pathogens

MDKT00210960 10x96 preps
EUR 2398.8

MagSi-S SH 1.0

MD03006 10 mL
EUR 483.6

MagSi-S SH 1.0

MD04006 100 mL
EUR 1548

MagSi-S SH 600

MD18006 10 mL
EUR 538.8

MagSi-S SH 600

MD19006 100 mL
EUR 1854

MagSi-S SH 3.0

MD43006 10 mL
EUR 483.6

MagSi-S SH 3.0

MD44006 100 mL
EUR 1548

MagSi-S NH2 1.0

MD01005 2 mL
EUR 73.2

MagSi-S NH2 1.0

MD03005 10 mL
EUR 204

Identification and Validation of Stage-Related PBMC Biomarkers in Breast Most cancers Utilizing MS-Primarily based Proteomics

Background: It’s well-described that the transcriptome of peripheral blood mononuclear cells (PBMCs) might be altered within the context of many malignancies to permit them keep away from the efficient immune response, which ends up in most cancers invasiveness. Right here, we used an MS-based technique to find biomarkers within the PBMCs of breast most cancers (BC) sufferers and validated them at completely different phases of BC.
Strategies: PBMCs had been remoted from the breast most cancers sufferers and had been cultured alone or co-cultured with breast most cancers cell traces. The function of PBMC within the invasion property of breast most cancers cells was explored. NF-kB exercise was additionally measured within the co-cultured breast most cancers cells. Identification of protein profiles within the secretome and proteome of the co-cultured PBMCs was carried out utilizing SWATH mass spectrometry. Pathway enrichment and gene ontology analyses had been carried out to search for the molecular pathways correlated with the protein expression profile of PBMCs within the breast most cancers sufferers. Quantitative real-time polymerase chain response (qPCR) was carried out to validate the candidate genes within the PBMC fraction of the breast most cancers sufferers on the major and metastatic phases. In silico survival evaluation was carried out to evaluate the potential scientific biomarkers in these PBMC subtypes.
Outcomes: PBMCs might considerably enhance the invasion property of the BC cells concomitant with a lower in E-cadherin and a rise in each Vimentin and N-cadherin expression. The NF-kB exercise within the BC cells considerably elevated following co-culturing implying the function of PBMCs in EMT induction. Enrichment evaluation confirmed that the differentially expressed proteins in PBMCs are primarily related to IL-17, PI3K-Akt, and HIF-1 signaling pathway, through which a set of seven proteins together with TMSB4X, HSPA4, S100A9, SRSF6, THBS1, CUL4A, and CANX had been incessantly expressed. Lastly, in silico evaluation confirmed {that a} gene set consisting of S100A9, SRSF6, THBS1, CUL4A, and CANX had been discovered to offer an perception for the identification of metastasis in breast most cancers sufferers.
Conclusion: In conclusion, our research revealed that the protein expression profile in PBMCs is a mirrored image of the proteins expressed within the BC tissue itself; nonetheless, the abundance degree is completely different because of the stage of most cancers.

Protein A/G Magnetic Beads

HY-K0202 1 mL
EUR 157.2

Protein A/G Magnetic Beads

EA-IP-007M 1mL
EUR 80
Description: Primary Antibodies

96 Magnetic Stand (For Magnetic Beads DNA/RNA Extraction/Magnetic Beads DNA/RNA Extraction)

MNS096-W each
EUR 70

Protein A/G/L Magnetic Beads

6547-1 each
EUR 405.6

Streptavidin Magnetic Beads

HY-K0208 5 mL
EUR 645.6

Streptavidin Magnetic beads

EA-IP-011M 1mL
EUR 241
Description: Primary Antibodies

Anti-HA magnetic beads

B26201 1 mL
EUR 579.6
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

Anti-HA magnetic beads

B26202 5 mL
EUR 1742.4
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

Anti-HA Magnetic Beads

HY-K0201 1 mL
EUR 334.8

SILICA MAGNETIC BEADS 1L

L5011001 1 liter
EUR 7260

Anti-Myc magnetic beads

B26301 1 mL
EUR 579.6
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Anti-Myc magnetic beads

B26302 5 mL
EUR 1742.4
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Anti-Flag magnetic beads

B26101 1 mL
EUR 579.6
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

Anti-Flag magnetic beads

B26102 5 mL
EUR 1742.4
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

DNA Cleanup Magnetic Beads

AG0311L 5 ml, 60 ml Ask for price
Description: DNA Cleanup Magnetic Beads are superparamagnetic, non-aggregating iron oxide particles (or ‘microspheres’) which can bind DNA ant high capcity. The highly efficient DNA purification magnetic beads are based clean-up system for the purification of DNA for NGS workflows. Superior yield, purity, and quality over the leading competitors, all at a better value, because we design and manufacture them in-house. DNA Cleanup Magnetic Beads enables faster binding kinetics, with high sensitivity & selectivity, in both manual and automated biomedical and research applications. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including PCR, qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping.

Anti-Flag Magnetic Beads

HY-K0207 1 mL
EUR 570

SILICA MAGNETIC BEADS 10ML

L5011010 10 ml
EUR 73

Magnetic Beads (DNA) 30 mL

P920-30 30 mL
EUR 399

Magnetic Beads (DNA) 450 mL

P920-450 450 mL
EUR 3999

Magnetic Beads (DNA) 5 mL

P920-5 5 mL
EUR 99

Magnetic Beads (DNA) 60 mL

P920-60 60 mL
EUR 699

Protein A/G Magnetic Beads for IP

B23201 1 ml
EUR 158.4
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A/G Magnetic Beads for IP

B23202 5 ml
EUR 558
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

BlackPearl® (Magnetic Beads)

20161006-1 - Ask for price

BlackPearl® (Magnetic Beads)

20161006-2 - Ask for price

BlackPearl® (Magnetic Beads)

20161006-3 - Ask for price

IP/CoIP Kit (Magnetic Beads)

EA-IP-K007M 50T
EUR 700
Description: Antibody Application Reagents

Magnetic Beads™ Streptavidin

SMB-B01 5mg
EUR 149.8
Description: The Streptavidin-Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin (SA). The beads can be used to capture the biotinylated proteins or other molecules, because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly.Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidin, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).The Streptavidin-Magnetic Beads is easy to capture the biotinylated proteins or other molecules in Chemiluminescence procedures, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Concanavalin A Magnetic beads

EA-IP-014M 1mL
EUR 159
Description: Primary Antibodies

Protein A BlackPearl® (Magnetic Beads)

20161000-1 - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-2 - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-3 - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-1 - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-2 - Ask for price

Anti-c-Myc Magnetic Beads

HY-K0206 5 mL
EUR 1368

Anti-tGFP magnetic beads, 1ml

TA150039 1 ml Ask for price

Anti-tGFP magnetic beads, 5ml

TA150040 5 ml Ask for price

9E10, Anti-Myc Magnetic beads

TA150044 1 ml Ask for price

9E10, Anti-Myc Magnetic beads

TA150045 5 ml Ask for price

Magnetic Beads PCR Cleanup Kit

MC004 each
EUR 3

Magnetic Beads PCR Cleanup Kit

MC048 each
EUR 25

Magnetic Beads PCR Cleanup Kit

MC096 each
EUR 45

Anti-HA (Nanobody) Magnetic Beads

AE109 500μL
EUR 403

Anti-GFP (Nanobody) Magnetic Beads

AE079 500μL
EUR 312

Anti-Myc (Nanobody) Magnetic Beads

AE107 1000μL
EUR 213.2

Human CD33-coupled Magnetic Beads

MBC-K008 2mg
EUR 2707.1
Description: Human CD33 protein is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259(Accession # P20138-1(R69G)).

Human CD38-coupled Magnetic Beads

MBC-K010 2mg
EUR 2707.1
Description: Human CD38 protein is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300(Accession # P28907-1).

Human EGFR-coupled Magnetic Beads

MBE-K012 2mg
EUR 2707.1
Description: Human EGFR protein is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645(Accession # P00533-1).

Human PSMA-coupled Magnetic Beads

MBP-K007 2mg
EUR 3349.1
Description: Human PSMA protein is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750(Accession # Q04609-1).

Human ROR1-coupled Magnetic Beads

MBR-K009 2mg
EUR 2707.1
Description: Human ROR1 protein is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403(Accession # Q01973-1).

Human BCMA-coupled Magnetic Beads

MBS-K004 2mg
EUR 2707.1
Description: Human BCMA protein is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54(Accession # Q02223-1).

Human CD19-coupled Magnetic Beads

MBS-K005 2mg
EUR 4312.1
Description: Human CD19 protein is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291(Accession # P15391-1).

Human HER2-coupled Magnetic Beads

MBS-K006 500ug
EUR 2835.5
Description: Human HER2 protein is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652(Accession # P04626-1).

Human ACE2-coupled Magnetic Beads

MBS-K013 2mg
EUR 2707.1
Description: Human ACE2 protein is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740(Accession # Q9BYF1-1).

Human CD73-coupled Magnetic Beads

MBS-K022 2mg
EUR 3349.1
Description: Human CD73 Protein is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549(Accession # P21589-1).

Human CD47-coupled Magnetic Beads

MBS-K023 2mg
EUR 2707.1
Description: Human CD47 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139(Accession # Q08722).

Human CD3E-coupled Magnetic Beads

MBS-K026 2mg
EUR 2707.1
Description: Human CD3E Protein is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126(Accession # P07766-1).

Human CD22-coupled Magnetic Beads

MBS-K052 2mg
EUR 4312.1
Description: Human CD22 Protein is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687(Accession # P20273-1).

Human DLL3-coupled Magnetic Beads

MBS-K053 2mg
EUR 2707.1
Description: Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.

Human uPAR-coupled Magnetic Beads

MBS-K076 2mg
EUR 2707.1
Description: Tumors are the result of uncontrolled proliferation of cells in different organs. Tumor development is a multistage process, that includes the generation of primary tumors, separation of tumors from primary sites, degradation of extracellular matrix (ECM), and distant metastasis of tumors. A variety of genes play important roles in the development of tumors, including the cell surface receptor urokinase-type plasminogen activator receptor (uPAR). uPAR is highly expressed in a variety of tumor cells, and a variety of signals regulated by uPAR play significant roles in tumor cell proliferation and metastasis, tumor-related glycolysis, the tumor microenvironment and angiogenesis. Studies have found that some specific drugs and antibodies have unique inhibitory effects on uPAR.

Magnetic Beads for DNA Purification

M1502-5 5 ml
EUR 332.4

DDDDK-Tag Antibody (Magnetic Beads)

abx005594-100l 100 µl
EUR 250

Amylose BlackPearl® (Magnetic Beads)

20161009-1 - Ask for price

Amylose BlackPearl® (Magnetic Beads)

20161009-2 - Ask for price

Human PCSK9-coupled Magnetic Beads

MBS-K054 2mg
EUR 2707.1
Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Human ASGR1-coupled Magnetic Beads

MBS-K055 2mg
EUR 2707.1
Description: ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2). ASGR1 has been shown to be efficiently targeted to the plasma membrane and to undergo constitutive endocytosis and recycling and found mainly expressed in the human liver.

Human ANGPT2-coupled Magnetic Beads

MBS-K035 2mg
EUR 2707.1
Description: Human ANGPT2 Protein is expressed from human 293 cells (HEK293). It contains AA Lys 275 - Phe 496(Accession # O15123-1).

Ni-NTA Magnetic Agarose Beads

20-512M1 2ml/Unit
EUR 116.39
Description: Suspension, 5% Magnetic Resin

Ni-NTA Magnetic Agarose Beads

20-512M2 5ml/Unit
EUR 263.6
Description: Suspension, 5% Magnetic Resin

Ni-NTA Magnetic Agarose Beads

20-512M3 10ml/Unit
EUR 477.13
Description: Suspension, 5% Magnetic Resin

Human VEGF165-coupled Magnetic Beads

MBS-K036 2mg
EUR 2707.1
Description: Human VEGF165 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191(Accession # P15692-4).

Human EGFRvIII-coupled Magnetic Beads

MBS-K020 2mg
EUR 2707.1
Description: Human EGFRvIII Protein is expressed from human 293 cells (HEK293). It contains AA Leu 25- Ser 378(Accession # NP_001333870.1).

Human HGF R-coupled Magnetic Beads

MBS-K021 2mg
EUR 2707.1
Description: Human HGF R Protein is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932(Accession # P08581).

Magnetic Beads for DNA size selection

AG0103 5 ml, 60 ml Ask for price
Description: The Magnetic Beads are ideal for DNA purification, concentration and size selection, along with next-generation sequencing library preparation kits.

Human CTLA-4 coupled Magnetic Beads

MBS-K042 2mg
EUR 2707.1
Description: Human CTLA-4 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162(Accession # Q6GR94-1).

Streptavidin BlackPearl® (Magnetic Beads)

20161004-1 - Ask for price

Streptavidin BlackPearl® (Magnetic Beads)

20161004-2 - Ask for price

Human Mesothelin-coupled Magnetic Beads

MBS-K024 2mg
EUR 2707.1
Description: Human Mesothelin Protein is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580(Accession # Q13421-3(R309P)).

Magnetic Beads DNA Extraction Kit (Food)

MF004 each
EUR 3

Magnetic Beads DNA Extraction Kit (Food)

MF048 each
EUR 55

Magnetic Beads DNA Extraction Kit (Food)

MF096 each
EUR 99

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-1 - Ask for price

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-2 - Ask for price

Human TROP-2-coupled Magnetic Beads

MBS-K028 2mg
EUR 2707.1
Description: Human TROP-2 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274(Accession # P09758-1).

Magnetic Beads DNA Extraction Kit (Blood)

MB004 each
EUR 3

Magnetic Beads DNA Extraction Kit (Blood)

MB048 each
EUR 40

Magnetic Beads DNA Extraction Kit (Blood)

MB096 each
EUR 75

Magnetic Beads DNA Extraction Kit (Plant)

MP004 each
EUR 3

Magnetic Beads DNA Extraction Kit (Plant)

MP048 each
EUR 55

Magnetic Beads DNA Extraction Kit (Plant)

MP096 each
EUR 99

Magnetic Beads Micro gDNA Extraction Kit

MM004 each
EUR 3

Magnetic Beads Micro gDNA Extraction Kit

MM048 each
EUR 63

Magnetic Beads Micro gDNA Extraction Kit

MM096 each
EUR 113

Anti-mCherry (Nanobody) Magnetic Beads Ab

AE094 1000μL
EUR 213.2

Human Nectin-4-coupled Magnetic Beads

MBS-K039 2mg
EUR 2707.1
Description: Human Nectin-4 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349(Accession # Q96NY8-1).