Beta-2-Transferrin Assay Testing

Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line #60540 _x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line #60541

Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TIGIT / NFAT Reporter - Jurkat Recombinant Cell Line, #60538 • CD155/TCR Activator - CHO Recombinant Cell Line, #60548

Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience cell lines._x000D_Optimized for use with Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway), #60520

Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including TrkA / SRE Reporter - HEK293 Recombinant Cell Line (#79798)

Description: Quantitativesandwich ELISA kit for measuring Human beta 2 transferrin (β2TF) in samples from serum, cerebrospinalfluid. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Description: Quantitativesandwich ELISA kit for measuring Human beta 2 transferrin (β2TF) in samples from serum, cerebrospinalfluid. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.

Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The RANK:RANKL TR-FRET Assay is designed to measure the inhibition of RANK binding to RANKL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated RANK, RANKL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Description: The PARP7 Chemiluminescent Activity Assay Kit is designed to measure PARP7 activity for screening and profiling applications. PARP7 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 384-well plate. Next, the PARP7 biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Description: The Papain-like Protease Assay Kit: Protease Activity is designed to measure PLPro activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.