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STAYBRITE KITS

Adenosine-5′-triphosphate (ATP) plays an important role in living systems. It has also been used as an indicator to monitor many biological processes and stages of diseases, etc. Accurate and reliable ATP level detection has wide applications. Luciferase/luciferin has been used to sensitively measure ATP levels. However, conventional luciferase/luciferin ATP detection systems are unstable. Luciferase loses activity rapidly. At BioVision, we have developed a highly stable luciferase, which is a genetically modified variant derived from the luciferase from Diaphanes pectineal (Chinese firefly) endemic to Yunnan province, China. We call the highly stable luciferase rLucHS.

Compared to the normal Photinus STAYBRITE KITS Pyralis phenotype, rLucHS provides improved stability, excellent sensitivity, and a wider and physiologically relevant effective pH range. At all pHs below ~ 8.2, Lucas is significantly more active than Photinus luciferase and is stable for weeks at room temperature and> 60 minutes at 37 ° C.

The activity profile shows a linear detection range for ATP values ​​as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5×10ᶦᶦRLU / mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.

We have developed a highly stable luciferase, which is a genetically modified variant derived from the luciferase from Diaphanes pectineal (Chinese firefly) endemic to Yunnan province, China. We call the highly stable luciferase rLucHS. Compared to the normal Photinus Pyralis phenotype, rLucHS provides improved stability, excellent sensitivity, and a wider and physiologically relevant effective pH range.

At all pHs below ~ 8.2, Lucas is significantly more active than Photinus luciferase and is stable for weeks at room temperature and> 60 minutes at 37 ° C. The activity profile shows a linear detection range for ATP values ​​as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5x10IIRLU / mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.